Effects of expression of mammalian G alpha and hybrid mammalian-yeast G alpha proteins on the yeast pheromone response signal transduction pathway.
Effects of expression of mammalian G alpha and hybrid mammalian-yeast G alpha proteins on the yeast pheromone response signal transduction pathway.
复制标题
哺乳动物Gα和杂交哺乳动物-酵母Gα蛋白的表达对酵母信息素响应信号转导途径的影响。
DOI：
10.1128/mcb.10.6.2582-2590.1990
复制
发表时间：
1990
影响因子：
5.3
通讯作者：
Tipper,DJ
中科院分区：
文献类型：
--
作者：
Kang,YS;Kane,J;Kurjan,J;Stadel,JM;Tipper,DJ
Scg1, the product of theSaccharomyces cerevisiae SCG1(also calledGPA1) gene, is homologous to the a subunits of G proteins involved in signal transduction in mammalian cells. Scg1 negatively controls the pheromone response pathway in haploid cells. Either pheromonal activation or anscg1null mutation relieves the negative control and leads to an arrest of cell growth in the G1 phase of the cell cycle. Expression of rat Gas was previously shown to complement the growth defect ofscg1null mutants while not allowing mating. We have extended this analysis to examine the effects of the short form of Gas (which lacks 15 amino acids present in the long form), Gαi2, Gαo, and Scg1-mammalian Gα hybrids. In addition, we have found that constructs able to complementscg1are also able to inhibit the response to pheromone and mating when expressed in a wild-typeSCG1strain. Overexpression of Scg1 has a similar inhibitory effect. These results are consistent with a model proposed for the action of Scg1 as the α component of a heterotrimeric G protein in which the βγ component (Ste4/Ste18) activates the pheromone response after dissociation from Scg1. They suggest that the Gα constructs able to complementscg1can interact with βγ to prevent activation of the pathway but are unable to interact with pheromone receptors to activate the pathway.
