Immunization with VP2 is sufficient for protection against lethal challenge with African horsesickness virus type 4
Immunization with VP2 is sufficient for protection against lethal challenge with African horsesickness virus type 4
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DOI：
10.1006/viro.1996.0304
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发表时间：
1996-06-01
期刊：
影响因子：
3.7
通讯作者：
Laegreid, WW
中科院分区：
文献类型：
--
作者：
StoneMarschat, MA;Moss, SR;Laegreid, WW
用含有非洲马病病毒4型(AHSV-4)L2基因片段的全长cDNA的痘苗构建体免疫马匹。所有免疫的马在用AHSV-4毒株攻击之前都产生了血清中和抗体。在挑战之前，没有出现酶标法反应的抗体。一组四匹血清阴性的对照马在出现典型的非洲马病肺部形式的临床症状和皮损后死亡，而接种疫苗的马临床正常。攻击后血清中和抗体滴度和ELISA反应抗体滴度的增加表明，在免疫的马中至少发生了一些攻击病毒的复制。这些结果表明，仅AHSV VP2就足以在马身上诱导保护性免疫反应，并表明ELISA反应性抗体在区分接种疫苗的马和自然暴露的马方面是有用的。(C)1996年学术出版社。
Horses were immunized by inoculation with a vaccinia construct containing a full-length cDNA corresponding to the L2 gene segment of African horsesickness virus type 4 (AHSV-4). All immunized horses developed serum neutralizing antibodies prior to challenge with Virulent AHSV-4. No ELISA-reactive antibodies were present prior to challenge. A group of four seronegative control horses died after developing clinical signs and lesions typical of the pulmonary form of African horsesickness while the immunized horses were clinically normal. Increases in serum neutralizing and ELISA-reactive antibody titers following challenge indicate that at least some replication of challenge virus occurred in immunized horses. These results demonstrate that AHSV VP2 alone is sufficient to induce a protective immune response in horses and indicate the usefulness of ELISA-reactive antibodies for differentiation of vaccinated and naturally exposed horses. (C) 1996 Academic Press, Inc.