Resident Endothelial Cells Surrounding Damaged Arterial Endothelium Reendothelialize the Lesion
Resident Endothelial Cells Surrounding Damaged Arterial Endothelium Reendothelialize the Lesion
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DOI：
10.1161/atvbaha.110.207365
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发表时间：
2010-09-01
影响因子：
8.7
通讯作者：
Suzuki, Norihiro
中科院分区：
文献类型：
--
作者：
Itoh, Yoshiaki;Toriumi, Haruki;Suzuki, Norihiro
评价剥脱软脑膜血管的内皮修复过程，以阐明重建内皮的机制（因为脑动脉损伤后的内皮修复对于预防血栓形成，维持血管张力，以及通过血脑屏障保护脑）。方法和结果-内皮细胞（EC）在350 μ m-光化学反应损伤大脑中动脉长段。Tie 2-绿色荧光蛋白转基因小鼠用于EC的鉴定。内皮损伤后6小时，将EC从受损动脉的管腔表面分离，然后用血小板毯覆盖。在24小时内，裸露的动脉在两个边缘开始恢复，EC伸长和迁移。近端边缘的修复率快于远端边缘。EC增殖的再内皮化在2 ~ 3天达到高峰，在5天结束，EC长度正常化，没有明显的外国祖细胞的参与。在再内皮化期间应考虑预防血栓形成、血管痉挛和治疗血脑屏障功能障碍。（Arterioscler Thromb Vasc Biol.2010; 30：1725-1732.）
Objective-To evaluate endothelial repair processes in denuded pial vessels to clarify mechanisms for reconstructing endothelium (because endothelial repair of the cerebral artery after its damage is critical for the prevention of thrombosis, the maintenance of vascular tone, and the protection of the brain by the blood-brain barrier).Methods and Results-Endothelial cells (ECs) in a 350-mu m-long segment of the middle cerebral artery were damaged through a photochemical reaction. Tie2-green fluorescent protein transgenic mice were used for the identification of ECs. Six hours after the endothelial damage, ECs were detached from the luminal surface of the damaged artery, which was then covered with a platelet carpet. Within 24 hours, recovery of the denuded artery started at both edges, with EC elongation and migration. The repair rate was faster at the proximal edge than at the distal edge. Reendothelialization with EC proliferation peaked at 2 to 3 days and ended at 5 days, together with normalization of EC length, with no apparent involvement of foreign progenitor cells.Conclusion-Our in vivo study demonstrated a stepwise reendothelialization process by resident ECs of the pial artery. The prevention of thrombosis, vasospasm, and treatment for blood-brain barrier dysfunction should be considered during the reendothelialization period. (Arterioscler Thromb Vasc Biol. 2010; 30: 1725-1732.)