Pattern and duration of the inhibitory effect of alcohol administered acutely on suckling-induced prolactin in lactating rats.
Pattern and duration of the inhibitory effect of alcohol administered acutely on suckling-induced prolactin in lactating rats.
复制标题
酒精对哺乳大鼠哺乳诱导催乳素的急性抑制作用的模式和持续时间。
DOI：
10.1111/j.1530-0277.1990.tb01244.x
复制
发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Bergeski,BA
中科院分区：
文献类型：
--
作者：
Subramanian,MG;Chen,XG;Bergeski,BA
我们已经表征了急性酒精给药对哺乳期大鼠哺乳诱导的催乳素（PRL）释放的抑制作用的模式和持续时间。在哺乳期第2天，将窝调整为8只幼仔。在第6天，母鼠植入心房导管，并在哺乳期第10天进行实验。在0800 h时将幼仔从母鼠中取出。在1300 h时将充满肝素化盐水的延长管连接到导管上。在1400 h时，取出输注前（PRE 0）血样，然后输注盐水（对照）或含酒精的盐水（0.5、1.0、2.0、2.5 g/kg体重剂量）溶液。在取出输注后（POST 0）血样后，将幼仔送回母体。随后在开始哺乳后10、30、60、120和180 min采集血样。在单独的组中，通过在输注生理盐水或含酒精的生理盐水后的PRE 0、POST 0和20、40、60、80、100和120 min采集血样，研究酒精对基础PRL的影响。然而，在哺乳10、30、60和120分钟时，以> 1.0 g/kg体重的剂量给予酒精，可抑制哺乳诱导的PRL。哺乳180分钟后，各组间血浆PRL水平相当。2.5 g/kg体重酒精组的哺乳潜伏期大于其他组，但在3小时哺乳期内消耗的乳汁量相当。1.0、2.0和2.5 g/kg/体重剂量组的血液酒精清除率（mg/kg/hr）分别为220 ± 16、342 ± 18和385 ± 24。通过哺乳期第2、7、14和21天的窝重来判断幼崽的生长速度，没有差异。数据表明酒精（急性给药）不会影响哺乳期大鼠的基础血浆催乳素。相反，哺乳诱导的PRL释放受到抑制，并且对于所用的最高酒精剂量（2.5 g/kg体重），这种抑制作用持续约2 h。此外，还观察到血液酒精浓度为65 mg/dl或更高是对哺乳诱导的PRL释放发挥抑制作用所必需的。
We have characterized the pattern and duration of the inhibitory effect of acute alcohol administration on suckling‐induced prolactin (PRL) release in the lactating rat. On day 2 of lactation, litters were adjusted to eight pups. On day 6, dams were implanted with an atrial catheter and experiments were conducted on day 10 of lactation. Pups were removed from the dams at 0800 hr. An extension tube filled with heparinized saline was attached to the catheter at 1300 hr. At 1400 hr, a preinfusion (PRE 0) blood sample was removed and was followed by infusion of saline (control) or alcohol in saline (0.5, 1.0, 2.0, 2.5 g/kg body weight doses) solutions. Following the removal of a postinfusion (POST 0) blood sample, pups were returned to the mother. Subsequent blood samples were obtained 10, 30, 60, 120, and 180 min after initiation of suckling. In separate groups, the effects of alcohol on basal PRL were studied by collecting blood samples PRE 0, POST 0 and 20,40,60,80,100, and 120 min following infusion of saline or alcohol in saline to lactating rats also separated from their pups for 6 hr.Alcohol infusion did not after basal PRL. However, suckling‐induced PRL was inhibited at 10, 30, 60, and 120 min of suckling by alcohol administered at doses > 1.0 g/kg body weight. After 180 min of suckling, plasma PRL levels were comparable among groups. The suckling latency for the 2.5 g/kg body weight alcohol group was greater than for other groups, but the quantities of milk consumed during the 3‐hr suckling period were comparable. The blood alcohol clearance rates expressed as mg/kg/hr were 220 ± 16, 342 ± 18, and 385 ± 24 for 1.0, 2.0, and 2.5 g/kg/body weight doses, respectively. The growth rate of the pups are judged by the litter weight on days 2, 7, 14, and 21 of lactation, did not differ.The data indicate that alcohol (administered acutely) does not affect the basal plasma PRL in lactating rats. In contrast, suckling‐induced PRL release is inhibited, and this inhibitory effect lasts approximately for 2 hr for the highest alcohol dose (2.5 g/kg body weight) employed. Further, it was also observed that a blood alcohol concentration of 65 mg/dl or greater is necessary to exert the inhibitory effect on suckling‐induced PRL release.