Cell inactivation and DNA single- and double-strand breaks in cultured mammalian cells irradiated by a thermal neutron beam.
Cell inactivation and DNA single- and double-strand breaks in cultured mammalian cells irradiated by a thermal neutron beam.
复制标题
受热中子束照射的培养哺乳动物细胞中的细胞失活和 DNA 单链和双链断裂。
DOI：
--
复制
发表时间：
1986
期刊：
影响因子：
--
通讯作者：
M. Akaboshi
中科院分区：
文献类型：
--
作者：
H. Maki;M. Saito;Tooru Kobayashi;K. Kawai;M. Akaboshi
研究了热中子辐照对HeLa细胞活力和DNA链断裂诱导修复动力学的影响，并与伽玛辐照进行了比较。热中子存活曲线没有初始肩。测定中子束的相对生物有效性（r.b.e）值对细胞杀伤（D0值比值）为2.2，对碱性沉降和碱性洗脱的单链断裂（ssb）分别为1.8和0.89，对中性洗脱的双链断裂（dsb）为2.6。热中子和伽马射线在ssb和dsb的修复动力学中没有观察到差异。结果表明，胞内核反应14N(n,p)14C诱导的效应是观察到高r.b.e.值的主要原因。
The effects on the cellular viability and induction and repair kinetics of DNA strand breaks in HeLa cells were examined after exposure to a thermal neutron beam and compared with those after gamma-irradiation. The thermal neutron survival curve had no initial shoulder. The relative biological effectiveness (r.b.e.) value of the neutron beam was determined to be 2.2 for cell killing (ratio of D0 values), 1.8 and 0.89 for single strand breakage (ssb) by alkaline sedimentation and alkaline elution respectively, and for double strand breakage (dsb) 2.6 by neutral elution. No difference was observed between thermal neutrons and gamma-rays in the repair kinetics of ssb and dsb. It is suggested that the effect induced by the intracellular nuclear reaction, 14N(n,p)14C is mainly responsible for the high r.b.e. values observed.