Temporary Solubilizing Tags Method for the Chemical Synthesis of Hydrophobic Proteins
Temporary Solubilizing Tags Method for the Chemical Synthesis of Hydrophobic Proteins
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DOI：
10.2174/1385272822666181211121758
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发表时间：
2019-03
影响因子：
2.6
通讯作者：
Dongdong Zhao;Xiao-Wen Fan;He Hao;Hong-Li Zhang;Ye Guo
中科院分区：
文献类型：
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作者：
Dongdong Zhao;Xiao-Wen Fan;He Hao;Hong-Li Zhang;Ye Guo
疏水蛋白作为细胞蛋白的一种，在维持细胞正常的生命周期中起着重要的作用。对疏水蛋白结构和功能的研究促进了对重大疾病病因的探索，以及开发新的疾病治疗药物。然而，疏水蛋白质的水溶性差给其制备、分离、表征和功能研究带来了困难。临时增溶标签被认为是有效解决疏水蛋白质水溶性差问题的实用策略。这种策略可以显著提高疏水肽/蛋白质的水溶性，使它们像水溶性肽/蛋白质一样易于纯化、表征。更重要的是，临时增溶标签可以在蛋白质合成后去除，因此疏水性蛋白质的结构和功能不受影响。目前，暂时增溶标签已被成功地应用于膜蛋白、脂蛋白、分子伴侣等重要疏水蛋白的制备。本文综述了近年来临时增溶标签的研究和应用。
Hydrophobic proteins, as one of the cellular protein classifications, play an essential function in maintaining the normal life cycle of living cells. Researches on the structure and function of hydrophobic proteins promote the exploration of the causes of major diseases, and development of new therapeutic agents for disease treatment. However, the poor water solubility of hydrophobic proteins creates problems for their preparation, separation, characterization and functional studies. The temporary solubilizing tags are considered a practical strategy to effectively solve the poor water solubility problem of hydrophobic proteins. This strategy can significantly improve the water solubility of hydrophobic peptides/proteins, making them like water-soluble peptides/proteins easy to be purified, characterized. More importantly, the temporary solubilizing tags can be removed after protein synthesis, so thus the structure and function of the hydrophobic proteins are not affected. At present, temporary solubilizing tags have been successfully used to prepare many important hydrophobic proteins such as membrane proteins, lipoproteins and chaperones. In this review, we summarize the recent researches and applications of temporary solubilizing tags.