Herpes simplex virus-mediated human hypoxanthine-guanine phosphoribosyltransferase gene transfer into neuronal cells.
Herpes simplex virus-mediated human hypoxanthine-guanine phosphoribosyltransferase gene transfer into neuronal cells.
复制标题
单纯疱疹病毒介导的人次黄嘌呤鸟嘌呤磷酸核糖转移酶基因转移到神经元细胞中。
DOI：
10.1128/mcb.8.1.457-460.1988
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发表时间：
1988
影响因子：
5.3
通讯作者：
Kelley,WN
中科院分区：
文献类型：
--
作者：
Palella,TD;Silverman,LJ;Schroll,CT;Homa,FL;Levine,M;Kelley,WN
嘌呤补救酶次黄嘌呤-鸟嘌呤磷酸核糖转移酶（HPRT）的几乎完全缺乏会导致一种毁灭性的神经系统疾病，Lesch-Nyhan综合征。在体外将HPRT基因转移到成纤维细胞和成淋巴细胞中以及在体内将HPRT基因转移到造血细胞中已经由其他小组用逆转录病毒衍生的载体完成。然而，似乎有必要将HPRT基因转移到神经元细胞中以纠正这种疾病的神经功能障碍。嗜神经病毒单纯疱疹病毒1型具有使其适合用作将HPRT基因转移到神经元组织中的载体的特征。本报告描述了一个HPRT缺陷的大鼠神经瘤细胞系，命名为B103-4C的分离，并含有人HPRT cDNA的重组单纯疱疹病毒1型的建设。这些重组病毒用于感染B103-4C细胞。感染的细胞表达HPRT活性，这是人类起源的。
The virtually complete deficiency of the purine salvage enzyme hypoxanthine-guanine phosphoribosyltransferase (HPRT) results in a devastating neurological disease, Lesch-Nyhan syndrome. Transfer of the HPRT gene into fibroblasts and lymphoblasts in vitro and into hematopoietic cells in vivo has been accomplished by other groups with retroviral-derived vectors. It appears to be necessary, however, to transfer the HPRT gene into neuronal cells to correct the neurological dysfunction of this disorder. The neurotropic virus herpes simplex virus type 1 has features that make it suitable for use as a vector to transfer the HPRT gene into neuronal tissue. This report describes the isolation of an HPRT-deficient rat neuroma cell line, designated B103-4C, and the construction of a recombinant herpes simplex virus type 1 that contained human HPRT cDNA. These recombinant viruses were used to infect B103-4C cells. Infected cells expressed HPRT activity which was human in origin.