TYPE-I PROTEIN KINASE-C ISOZYME IN THE VISUAL-INFORMATION-PROCESSING PATHWAY OF MONKEY BRAIN
TYPE-I PROTEIN KINASE-C ISOZYME IN THE VISUAL-INFORMATION-PROCESSING PATHWAY OF MONKEY BRAIN
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DOI：
10.1002/jcb.240390406
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发表时间：
1989-04-01
影响因子：
4
通讯作者：
HUANG, KP
中科院分区：
文献类型：
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作者：
HUANG, FL;YOSHIDA, Y;HUANG, KP
以前我们使用PKC同工酶特异性抗体进行免疫印迹分析，我们证明了PKC同工酶在猴子和大鼠大脑的不同区域中的异质性分布，并且I型PKC在小脑、海马体、杏仁核和大脑皮层中最丰富(Huang等：J.Biol Chem262：15714-15720,1987年)。使用这些抗体，我们还证明了I、II和III型PKC分别是PKC基因γ、β和α的产物(Huang等人：Biochem BiPhys Commun 149：946-952,1987)。免疫细胞化学分析显示，I型PKC特异性抗体在海马结构、杏仁核、小脑和新皮质的不同类型神经元上呈强阳性反应。在海马区，齿状回颗粒细胞和海马区锥体细胞染色较深。通过免疫印迹分析，测定了参与视觉信息处理和存储的猴大脑皮质多个区域的PKC同工酶的相对水平。III型和III型PKC均呈均匀分布，中等水平，I型PKC沿枕叶皮质向颞叶再到边缘呈递增趋势。神经行为学研究表明，与纹状体、纹状体前区和后颞区相比，前、内侧颞区的新皮质和边缘区域更直接地参与视觉表征的存储，而且海马体和杏仁核在记忆形成中都很重要。由于I型PKC在海马区、杏仁核和前颞叶高水平表达，我们预测I型蛋白激酶C可能参与了记忆功能的重要可塑性变化。
Previously using PKC isozyme-specific antibodies for immunoblot analysis, we demonstrated the heterogeneous distribution of PKC isozymes in various regions of monkey and rat brains and that type I PKC was most abundant in cerebellum, hippocampus, amygdala, and cerebral cortex (Huang et al.: J. Biol Chem 262:15714-15720, 1987). Using these antibodies, we have also demonstrated that type I, II, and III PKC are products of PKC genes .gamma., .beta., and .alpha., respectively (Huang et al.: Biochem Biophys Res Commun 149:946-952, 1987). By immunocytochemical analysis, type I PKC-specific antibody showed strong reactivity in various types of neuron in hippocampal formation, amygdala, cerebellum, and neocortex. In hippocampal formation, granule cells of dentate gyrus and pyramidal cells of hippocampus were heavily stained. By immunoblot analysis, relative levels of PKC isozymes in several areas of monkey cerebral cortex involved in the visual information processing and storage were determined. Both type II and III PKCs appeared to be evenly distributed and at moderate levels, type I PKC formed a gradient of increasing concentration rostral along the cerebral cortex of occipital to temporal and then to the limbic areas. Neurobehavioral studies have demonstrated that the neocortical and limbic areas of the anterior and medial temporal regions participate more directly than the striate, prestriate, and posterior temporal regions in the storage of visual representations and that both hippocampus and amygdala are important in the memory formation. As type I PKC is present at high levels in hippocampus, amygdala, and anterior temporal lobe, we predict that the type I protein kinase C may participate in the plastic changes important for mnemonic function.