COMPARISON OF CYTOKINE LEVELS AND EMBRYO TOXICITY IN PERITONEAL-FLUID IN INFERTILE WOMEN WITH UNTREATED OR TREATED ENDOMETRIOSIS
COMPARISON OF CYTOKINE LEVELS AND EMBRYO TOXICITY IN PERITONEAL-FLUID IN INFERTILE WOMEN WITH UNTREATED OR TREATED ENDOMETRIOSIS
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DOI：
10.1016/s0002-9378(11)91672-x
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发表时间：
1992-07-01
影响因子：
9.8
通讯作者：
MIZUNO, M
中科院分区：
文献类型：
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作者：
TAKETANI, Y;KUO, TM;MIZUNO, M
目的：我们的目的是研究腹膜液中细胞因子水平与其胚胎毒性之间的关系。研究设计：对未发生子宫内膜异位症（n = 21）、未发生子宫内膜异位症（n = 19）和因子宫内膜异位症接受药物治疗（n = 10）的不孕妇女的腹膜液中白细胞介素-1和肿瘤坏死因子的水平进行了测量。在含有不同浓度腹膜液的培养基中，研究了小鼠双细胞胚胎与输卵管共培养的胚胎毒性。结果：未经治疗的子宫内膜异位症患者的腹膜液中细胞因子水平明显高于未患子宫内膜异位症的女性，但接受过达那唑或布瑟林治疗的女性的细胞因子水平极低。未经治疗的子宫内膜异位症妇女的腹膜液对小鼠双细胞胚胎的卵裂有不利影响。经医学治疗后，腹膜液的胚胎毒性几乎检测不出来。结论：本研究结果为提高子宫内膜异位症不孕妇女生殖能力的药物治疗提供了一定的理论依据。
OBJECTIVE: Our aim was to examine the relationship between the levels of cytokines in peritoneal fluid and its embryo toxicity.STUDY DESIGN: The levels of interleukin-1 and tumor necrosis factor were measured in peritoneal fluid from infertile women who did not have endometriosis (n = 21), who had untreated endometriosis (n = 19), and who had undergone medical treatment for endometriosis (n = 10). Embryo toxicity was investigated in mouse two-cell embryos cocultured with the oviducts in culture media that contained various concentrations of peritoneal fluid.RESULTS: The levels of cytokines were significantly higher in the peritoneal fluid from women who had untreated endometriosis than in women who did not have endometriosis, but they were extremely low in women who had undergone medical treatment with either danazol or buserelin. The peritoneal fluid from women who had untreated endometriosis adversely affected the cleavage of mouse two-cell embryos. After medical treatment the embryo toxicity of the peritoneal fluid was almost undetectable.CONCLUSION: These results offer some theoretic bases in support of medical treatment to improve reproductive performance in infertile women who have endometriosis.