Increased IL‐10 production by stimulated whole blood cultures in primary IgA nephropathy
Increased IL‐10 production by stimulated whole blood cultures in primary IgA nephropathy
复制标题
原发性 IgA 肾病中刺激全血培养可增加 IL-10 的产生
DOI：
10.1046/j.1365-2249.1998.00506.x
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发表时间：
1998
影响因子：
4.6
通讯作者：
Van Kooten
中科院分区：
文献类型：
--
作者：
DE Fijter;Daha;Schroeijers;Van Es;Van Kooten
大多数原发性 IgA 肾病 (IgAN) 患者的骨髓中产生 IgA1 的 B 淋巴细胞的记忆能力明显较高，血浆 IgA1 水平也较高。粘膜免疫系统和骨髓室之间的连接可能基于抗原呈递细胞（APC）或抗原特异性淋巴细胞的运输。细胞因子在淋巴细胞的增殖和分化中发挥重要作用。为了尽可能模拟体内情况，我们使用脂多糖 (LPS) 或植物血凝素 (PHA) 刺激的全血 (WB) 培养物，评估了 23 名 IgAN 患者和匹配对照组的离体细胞因子产生情况。通过细胞因子特异性 ELISA 测定培养物上清液中干扰素-γ (IFN-γ)、IL-2、IL-6、IL-10 和肿瘤坏死因子-α (TNF-α) 的产生。与对照组相比，PHA刺激的培养物导致IgAN患者的IL-10（P < 0.001）、IL-2（P < 0.005）和IFN-γ（P < 0.001）水平显着升高，但TNF-α或IL-6水平没有发现显着差异。在 LPS 刺激的培养物中，两组之间唯一的显着差异 (P < 0.001) 是 IgAN 患者 IL-10 产量的增加。受刺激的 WB 培养物中细胞因子产生的增强表明 IgAN 患者的单核细胞相关 T 细胞反应发生了改变。 IL-10 产量的增加最终可能导致骨髓中产生 IgA 的 B 淋巴细胞数量增加。此外，高水平的内源性 IL-10 可能会下调单核细胞或一般 APC 的效应功能，从而下调粘膜水平的 IgA 反应。
Most patients with primary IgA nephropathy (IgAN) have a significantly higher memory repertoire of IgA1‐producing B lymphocytes in their bone marrow together with high plasma levels of IgA1. The connection between the mucosal immune system and the bone marrow compartment is probably based on traffic of either antigen‐presenting cells (APC) or antigen‐specific lymphocytes. Cytokines play an important role in the proliferation and differentiation of lymphoid cells. In order to mimic the in vivo situation as much as possible, we assessed cytokine production profiles ex vivo in 23 IgAN patients and matched controls, using lipopolysaccharide (LPS)‐ or phytohaemagglutinin (PHA)‐stimulated whole blood (WB) cultures. Interferon‐gamma (IFN‐γ), IL‐2, IL‐6, IL‐10 and tumour necrosis factor‐alpha (TNF‐α) production in culture supernatants were determined by cytokine‐specific ELISAs. Compared with controls, PHA‐stimulated cultures resulted in significantly higher IL‐10 (P < 0.001), IL‐2 (P < 0.005) and IFN‐γ (P < 0.001) levels in IgAN patients, but no significant differences in TNF‐α or IL‐6 levels were found. In LPS‐stimulated cultures, the only significant difference (P < 0.001) between the two groups was the increased IL‐10 production in IgAN patients. The enhanced cytokine production in stimulated WB cultures suggests altered monocyte‐related T cell responses in patients with IgAN. Increased IL‐10 production may eventually result in an increased number of IgA‐producing B lymphocytes in the bone marrow. In addition, high levels of endogenous IL‐10 may down‐regulate the effector functions of monocytes, or possibly APC in general, and consequently the IgA response at the mucosal level.
人外周血中有丝分裂原刺激的 T 细胞和 B 细胞反应所必需的辅助细胞的表型：通过其对溶酶体药物 L-亮氨酸甲酯的敏感性进行划分。
DOI：
--
发表时间：
1983
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Thiele,DL;Kurosaka,M;Lipsky,PE
通讯作者：
Lipsky,PE
来自 IgA 肾病患者和一级亲属的 B 淋巴细胞经 Epstein-Barr 病毒转化，导致免疫球蛋白合成增加，而不仅限于 IgA。
DOI：
10.1016/s0272-6386(12)80251-4
发表时间：
1991
期刊：
American journal of kidney diseases : the official journal of the National Kidney Foundation
影响因子：
--
作者：
Jackson,S;Galla,JH;Kirk,KA;Thorn,BT;Julian,BA
通讯作者：
Julian,BA