Phosphate starvation responsive GmSPX5 mediates nodule growth through interaction with GmNF-YC4 in soybean (Glycine max)
Phosphate starvation responsive GmSPX5 mediates nodule growth through interaction with GmNF-YC4 in soybean (Glycine max)
复制标题
磷酸盐饥饿响应 GmSPX5 通过与大豆 (Glycine max) 中的 GmNF-YC4 相互作用介导根瘤生长
DOI：
10.1111/tpj.15520
复制
发表时间：
2021
期刊：
影响因子：
7.2
通讯作者：
Tian Jiang
中科院分区：
文献类型：
--
作者：
Zhuang Qingli;Xue Yingbin;Yao Zhufang;Zhu Shengnan;Liang Cuiyue;Liao Hong;Tian Jiang
Phosphorus (P) deficiency adversely affects nodule development as reflected by reduced nodule fresh weight in legume plants. Though mechanisms underlying nodule adaptation to P deficiency have been studied extensively, it remains largely unknown which regulator mediates nodule adaptation to P deficiency. In this study, GUS staining and quantitative reverse transcription‐PCR analysis reveal that theSPXmemberGmSPX5is preferentially expressed in soybean (Glycine max) nodules. Overexpression ofGmSPX5enhanced soybean nodule development particularly under phosphate (Pi) sufficient conditions. However, the Pi concentration was not affected in soybean tissues (i.e., leaves, roots, and nodules) ofGmSPX5overexpression or suppression lines, which distinguished it from other well‐knownSPXmembers functioning in control of Pi homeostasis in plants. Furthermore, GmSPX5 was observed to interact with the transcription factor GmNF‐YC4in vivoandin vitro. Overexpression of eitherGmSPX5orGmNF‐YC4significantly upregulated the expression levels of five asparagine synthetase‐related genes (i.e.,GmASL2–6) in soybean nodules. Meanwhile, yeast one‐hybrid and luciferase activity assays strongly suggested that interactions of GmSPX5 and GmNF‐YC4 activateGmASL6expression through enhancing GmNF‐YC4 binding of theGmASL6promoter. These results not only demonstrate the GmSPX5–GmNF‐YC4–GmASL6 regulatory pathway mediating soybean nodule development, but also considerably improve our understanding of SPX functions in legume crops.
