The effects of ethanol on dopaminergic neurons of the ventral tegmental area studied with intracellular recording in brain slices
The effects of ethanol on dopaminergic neurons of the ventral tegmental area studied with intracellular recording in brain slices
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DOI：
10.1111/j.1530-0277.1998.tb03644.x
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发表时间：
1998-02-01
影响因子：
3.2
通讯作者：
Appel, SB
中科院分区：
文献类型：
--
作者：
Brodie, MS;Appel, SB
蔡氏腹侧被盖区（VTA）的多巴胺能神经元与乙醇及许多其他滥用药物的奖赏效应的介导有关。我们先前在脑切片上进行的细胞外研究表明，乙醇会提高VTA中多巴胺能神经元的放电频率。在当前的细胞内研究中，乙醇（40 - 160 mM）提高了大多数（77%）VTA神经元的自发放电频率。此外，大多数（75%）VTA神经元会因乙醇而去极化。乙醇还改变了VTA神经元自发动作电位的形状，降低了峰后超极化的幅度（在74%的神经元中），并且也降低了动作电位去极化相的幅度（在86%接受测试的神经元中）。此外，对有乙醇和无乙醇情况下的电压/电流曲线的分析表明，乙醇在接近静息膜电位时对细胞膜电阻影响很小，但增强了在更超极化膜电位下激活的时间依赖性内向整流（I - h）。这项细胞内研究确定了乙醇的几种电生理效应，这些效应可能是乙醇诱导VTA神经元兴奋的基础，因此可能对乙醇的奖赏效应很重要。
Dopaminergic neurons in the ventral tegmental area of Tsai (VTA) have been implicated in the mediation of the rewarding effects of ethanol and many other drugs of abuse, Our previous extracellular studies in brain slices have demonstrated that ethanol increases the firing rate of dopaminergic neurons in the VTA, In the present intracellular study, ethanol (40-160 mM) increased the spontaneous firing rate of most (77%) VTA neurons. In addition, most (75%) VTA neurons were depolarized by ethanol, Ethanol also changed the shape of the spontaneous action potential in VTA neurons, reducing the amplitude of the spike after-hyperpolarization (in 74% of neurons) and also reducing the amplitude of the depolarizing phase of the action potential (in 86% of neurons tested), Furthermore, analysis of Voltage/Current curves in the presence and absence of ethanol showed that ethanol had little effect on the resistance of the cell membrane at membrane potentials near rest, but enhanced the time-dependent inward rectification activated at more hyperpolarized membrane potentials (I-h), This intracellular study identifies several electrophysiological effects of ethanol that may underlie the ethanol-induced excitation of VTA neurons and, therefore, may be important for the rewarding effects of ethanol.