Monodispersed spherical shaped selenium nanoparticles (SeNPs) synthesized by Bacillus subtilis and its toxicity evaluation in zebrafish embryos
Monodispersed spherical shaped selenium nanoparticles (SeNPs) synthesized by Bacillus subtilis and its toxicity evaluation in zebrafish embryos
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DOI：
10.1088/2053-1591/aaabeb
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发表时间：
2018-02-01
影响因子：
2.3
通讯作者：
Muthukumaran, Azhaguchamy
中科院分区：
文献类型：
--
作者：
Chandramohan, Subburaman;Sundar, Krishnan;Muthukumaran, Azhaguchamy
硒是人体必需的抗氧化和降血脂的元素之一。自然地，硒离子被代谢并转化为纳米硒。纳米粒子在治疗领域的应用越来越受到人们的关注。在本研究中，枯草芽孢杆菌用于将亚硒酸钠转化为SeNPs。采用傅里叶变换红外光谱（FTIR）、X射线衍射（XRD）、扫描电子显微镜（SEM）和能量色散X射线能谱（EDX）对合成的纳米硒进行了表征。SeNP的存在通过红色的形成来证实。红外光谱分析表明，细菌蛋白质在3562 cm（-1）和1678 cm（-1）处有明显的吸收峰，表明细菌蛋白质参与了亚硒酸钠的还原反应。SeNPs的平均尺寸为334 nm，形状为球形，分布均匀。XRD数据证实SeNPs本质上是无定形的。SeNP的zeta电位在带电荷时为负，这表明高稳定性。在本研究中，使用斑马鱼胚胎来研究SeNPs的毒性，结果表明，浓度超过10 μ g/ml（-1）会导致胚胎/幼体的毒性作用。较低浓度的SeNP可用于各种生物医学应用。
Selenium is one of the essential elements involved in antioxidative and antiinflammatory effects in human body. By naturally, selenium ions are metabolised and converted into nano selenium. Now a days there is an increasing attention on applications of nanoparticles in therapeutic field. In the present study Bacillus subtilis was used to convert sodium selenite to SeNPs. The synthesized SeNPs were characterized by Fourier Transform Infrared Spectroscopy (FTIR), X Ray Diffraction (XRD) and Scanning Electron Microscopy (SEM) coupled with Energy Dispersive X ray spectroscopy (EDX). The presence of SeNPs was confirmed by the formation of red colour. The bands were sharp with broad absorption peaks at 3562 cm(-1) and 1678(-1) cm in FTIR which showed that the bacterial proteins were responsible for the reduction of sodium selenite to SeNPs. The average size of the SeNPs was 334 nm and were spherical in shape with uniform distribution. The XRD data confirmed that SeNPs were of amorphous in nature. The zeta potential of SeNPs was negative in charge which indicated high stability. In the present study zebrafish embryos were used to study the toxicity of SeNPs and the results showed that the concentration beyond 10 mu g ml(-1) leads to toxic effects in embryos/hatchlings. The lesser concentration of SeNPs can be useful in various biomedical applications.