Effects of smoking cessation on biological monitoring markers in urine
Effects of smoking cessation on biological monitoring markers in urine
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DOI：
10.1186/s41021-020-00165-z
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发表时间：
2020-09-11
影响因子：
1.7
通讯作者：
Kawai, Kazuaki
中科院分区：
文献类型：
--
作者：
Kawasaki, Yuya;Li, Yun-Shan;Kawai, Kazuaki
尿尼古丁和可替宁水平通常作为烟草烟雾暴露的生物标志物进行测量。然而，这些生物标志物不适合评估戒烟几天的效果，因为它们的半衰期很短。在这项研究中，我们重点关注了55名戒烟计划患者尿中4-（甲基亚硝胺）-1-（3-吡啶基）-1-丁醇（NNAL）水平的变化，因为半衰期长。结果：在完成戒烟计划的受试者中（55例中有18例），在第一次就诊后8周，尿中尼古丁和可替宁水平分别下降到1.7%和0.2%。相比之下，戒烟8周后NNAL水平下降至12.3%。在同一时期，通过排除戒烟计划期间NNAL水平升高的受试者，尿m（7）Gua水平显著降低，从27.32 μ g/mg肌酐降至14.17 μ g/mg肌酐。8-OHdG水平也在同一时期内降低，但无显著差异。结论：NNAL可作为评价戒烟患者吸烟状况的一个较好的暴露指标。尿可替宁和NNAL水平与m（7）Gua水平呈正相关。
Introduction: Urinary nicotine and cotinine levels are often measured as biomarkers for tobacco smoke exposure. However, these biomarkers are not appropriate to evaluate the effects of quitting smoking for several days, because of their short half-lives. In this study, we focused on the changes in the urinary 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol (NNAL) levels of 55 patients in a smoking cessation program, because of the long half-life. At the same time, urinary 7-methylguanine (m(7)Gua) and 8-hydroxy-2 '-deoxyguanosine (8-OHdG), as DNA damage markers of cigarette smoking, were also measured.Results: In the subjects who completed the quit-smoking program (18 subjects out of 55), the urinary nicotine and cotinine levels decreased to 1.7 and 0.2% at 8 weeks after the first visit to the clinic. By contrast, the NNAL levels decreased to 12.3% at 8 weeks after quitting smoking. During the same period, the urinary m(7)Gua levels significantly decreased, from 27.32 mu g/mg creatinine to 14.17 mu g/mg creatinine by the elimination of subjects who showed increased levels of NNAL during the smoking cessation program. The 8-OHdG levels were also reduced within the same period, but were not significantly different. From the all data analysis, the urinary levels of cotinine and NNAL positively correlated with the level of m(7)Gua.Conclusions: NNAL may be an appropriate exposure marker for evaluating the smoking status of patients in a smoking cessation program. The urinary cotinine and NNAL levels positively correlated with the m(7)Gua levels.