Improvement of Hen Egg White lysozyme Crystal Quality by Control of Dehydration Process
Improvement of Hen Egg White lysozyme Crystal Quality by Control of Dehydration Process
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控制脱水过程提高鸡蛋清溶菌酶晶体质量
DOI：
10.1021/acs.cgd.9b01084
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发表时间：
2019
影响因子：
3.8
通讯作者：
Kenichi Kojima
中科院分区：
文献类型：
--
作者：
Haruhiko Koizumi;Satoshi Uda;Katsuo Tsukamoto;Kenji Hanada;Ryo Suzuki;Masaru Tachibana;Kenichi Kojima
在楔形边的锥形四方蛋清(HEW)溶菌酶晶体中，清晰地观察到由Pendellö̈Sung效应引起的等厚度条纹，表明亚晶之间的取向偏差约为10-4°。这归因于对从溶液中生长晶体的脱水过程的控制，这一过程起着重要的作用。通过调节脱水过程，提高了晶体的局部质量和整体的均匀性。在晶体中相同厚度区域的X射线形貌中观察到了振荡对比度。然而，这种对比的面积非常小，这表明存在缺陷，即使在晶体中可以观察到等厚度的条纹。我们还讨论了亚晶间有10~(-4)°错位的四方HEW溶菌酶晶体振荡对比度小的原因。
Equal-thickness fringes that are attributed to the Pendellö̈sung effect were clearly observed in the region of a tapered tetragonal hen egg white (HEW) lysozyme crystal with a wedgelike edge, which indicates that the misorientation between subgrains was on the order of 10–4°. This is attributed to control of the dehydration process for crystal growth from solution, which plays an important role. Both the local quality and whole homogeneity of the crystals were improved by regulating the dehydration process. Oscillatory contrast was observed in X-ray topographs from regions of the same thickness in the crystals. However, the area of such a contrast was quite small, which indicates the presence of defects, even in crystals for which equal-thickness fringes could be observed. We also discuss why the area of oscillatory contrast was small for tetragonal HEW lysozyme crystals with misorientations between subgrains on the order of 10–4°.