NO PRIMEXINE AND PLASMA MEMBRANE UNDULATION Is Essential for Primexine Deposition and Plasma Membrane Undulation during Microsporogenesis in Arabidopsis
NO PRIMEXINE AND PLASMA MEMBRANE UNDULATION Is Essential for Primexine Deposition and Plasma Membrane Undulation during Microsporogenesis in Arabidopsis
复制标题
拟南芥小孢子发生过程中 Primexine 沉积和质膜波动没有 Primexine 和质膜波动所必需
DOI：
10.1104/pp.111.184853
复制
发表时间：
2012-01-01
期刊：
影响因子：
7.4
通讯作者：
Yang, Zhong-Nan
中科院分区：
文献类型：
--
作者：
Chang, Hai-Shuang;Zhang, Cheng;Yang, Zhong-Nan
原胺沉积和质膜波动是花粉壁形成的初始步骤。然而，人们对参与这一重要生物过程的基因知之甚少。在这里，我们报道了一个新的基因，NO PRIMEXINE和质膜波动（NPU），它在拟南芥花粉壁发育的早期阶段起作用。NPU功能的丧失导致雄性不育，这是由于NPU突变体的胼胝质合成和孢粉素沉积缺陷造成的。透射电镜观察表明，npu突变体完全不存在原胺沉积和质膜波动。NPU编码一种具有两个跨膜结构域和一个胞内结构域的膜蛋白。原位杂交分析表明，在四分体时期，NPU在小孢子和绒毡层中强烈表达。这些结果表明，NPU在花粉壁发育早期的primesine沉积和质膜波动中起重要作用。
Primexine deposition and plasma membrane undulation are the initial steps of pollen wall formation. However, little is known about the genes involved in this important biological process. Here, we report a novel gene, NO PRIMEXINE AND PLASMA MEMBRANE UNDULATION (NPU), which functions in the early stage of pollen wall development in Arabidopsis (Arabidopsis thaliana). Loss of NPU function causes male sterility due to a defect in callose synthesis and sporopollenin deposition, resulting in disrupted pollen in npu mutants. Transmission electronic microscopy observation demonstrated that primexine deposition and plasma membrane undulation are completely absent in the npu mutants. NPU encodes a membrane protein with two transmembrane domains and one intracellular domain. In situ hybridization analysis revealed that NPU is strongly expressed in microspores and the tapetum during the tetrad stage. All these results together indicate that NPU plays a vital role in primexine deposition and plasma membrane undulation during early pollen wall development.