5'-Peptidyl substituents allow a tuning of the affinity of oligodeoxyribonucleotides for RNA.
5'-Peptidyl substituents allow a tuning of the affinity of oligodeoxyribonucleotides for RNA.
复制标题
5-肽基取代基可以调节寡脱氧核糖核苷酸对 RNA 的亲和力。
DOI：
10.1016/s0960-894x(98)00449-1
复制
发表时间：
1998
影响因子：
2.7
通讯作者：
Richert,C
中科院分区：
文献类型：
--
作者：
Sarracino,DA;Steinberg,JA;Vergo,MT;Woodworth,GF;Tetzlaff,CN;Richert,C
通过 UV 熔解分析测量酰胺连接的 5'-氨酰基和 5'-二肽基 DNA 八聚体与具有 3'-突出端的两个 RNA 十一聚体的亲和力。观察到熔点的序列依赖性增加。在低离子强度下，两个附加的赖氨酸残基比两个附加的 A:U 碱基对提高了熔点。
The affinity of amide-linked 5′-aminoacyl and 5′-dipeptidyl DNA octamers for two RNA undecamers with 3′-overhangs was measured via UV melting analysis. A sequence-dependent increase in melting points was observed. At low ionic strength, two appended lysine residues elevate melting points more than two additional A:U base pairs.