Iodide Induces Transforming Growth Factor Beta1 (TGF-β1) mRNA in Sheep Thyroid Cells
Iodide Induces Transforming Growth Factor Beta1 (TGF-β1) mRNA in Sheep Thyroid Cells
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碘化物诱导绵羊甲状腺细胞中转化生长因子 Beta1 (TGF-β1) mRNA
DOI：
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
G. Burrow
中科院分区：
文献类型：
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作者：
R. Yuasa;M. Eggo;J. Meinkoth;W. Dillmann;G. Burrow
我们检测了原代绵羊甲状腺细胞培养物中TGF-β mRNA的水平，以确定碘对甲状腺细胞的抑制作用是否可以通过诱导TGF-β mRNA来解释，以及这种诱导是否由碘有机化介导。甲状腺细胞与TSH和五种添加剂（胰岛素、生长激素抑制素、生长激素、转铁蛋白和甘氨酰-L-组氨酰-L-溶素）一起孵育2-3周，然后暴露于碘化钠（Nal）或1-甲基咪唑-2-硫醇（甲巯咪唑，MMI）或两者72小时。用大鼠TGF-β1 cDNA探针进行北方印迹分析，发现10- 6 M和10-4 M的碘化物显著增加TGF-β mRNA的量。这种TGF-β1 mRNA的增加被有机化抑制剂甲巯咪唑所消除。这些数据表明，碘对甲状腺生长和功能的影响可能是通过一个过程介导的，该过程涉及碘的有机化和TGF-β1 mRNA水平的增加。
We examined TGF-β mRNA levels in primary sheep thyroid cell cultures to determine whether the inhibitory effects of iodide on thyroid cells could be explained by an induction of TGF-β mRNA and if this induction was mediated by iodine organification. Thyroid cells were incubated with TSH and five additives (insulin, somatostatin, growth hormone, transferrin, and glycyl-L-histidyl-L-lysin) for 2-3 weeks and then were exposed to sodium iodide (Nal) or 1-methylimidazole-2-thiol (methimazole, MMI), or both for 72 h. Iodide at 10-6M and 10-4 M significantly increased the amount of TGF-β mRNA as determined by Northern blot analysis with a rat TGF-β1 cDNA probe. This increase in TGF-β1 mRNA was abolished'by the addition of methimazole, an inhibitor of organification. These data indicate that the effects of iodide on thyroid growth and function may be mediated by a process that involves organification of iodide and increases in TGF-β1 mRNA levels.
影响因子：
--
作者：
G. Pfeifer;A. Riggs
通讯作者：
G. Pfeifer;A. Riggs
碘抑制培养物中大鼠甲状腺细胞的增殖。
影响因子：
4.8
作者：
Tramontano,D;Veneziani,BM;Lombardi,A;Villone,G;Ingbar,SH
通讯作者：
Ingbar,SH
绵羊甲状腺细胞中的胰岛素样生长因子：作用、受体和产生。
DOI：
10.1016/0006-291x(88)90219-7
发表时间：
1988
影响因子：
3.1
作者：
Bachrach,LK;Eggo,MC;Hintz,RL;Burrow,GN
通讯作者：
Burrow,GN