Circulating and brain BDNF levels in stroke rats. Relevance to clinical studies.
Circulating and brain BDNF levels in stroke rats. Relevance to clinical studies.
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DOI：
10.1371/journal.pone.0029405
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Marie C
中科院分区：
文献类型：
--
作者：
Béjot Y;Mossiat C;Giroud M;Prigent-Tessier A;Marie C
脑源性神经营养因子（BDNF）水平是在中风动物模型的脑中测量的，而中风患者的神经营养因子水平是在血浆或血清样品中测量的。本研究旨在探讨脑卒中患者循环BDNF水平的意义。通过将不同数量的微球注射到颈动脉循环中以模拟在中风患者中观察到的不同程度的中风严重性，在大鼠中诱导单侧缺血性中风。于栓塞前、栓塞后4 h、24 h和8 d从颈静脉连续采血，并于栓塞后4 h、24 h和8 d取全脑。然后根据栓塞程度选择大鼠，因此不同时间点大鼠中风严重程度的分布较大但相似。使用ELISA试验，测定选定大鼠血浆、血清和脑中的BDNF水平。而血浆和血清BDNF水平没有改变中风，中风诱导脑BDNF水平的增加，在4小时和24小时后栓塞，这是不相关的中风严重程度。个体血浆BDNF水平与脑水平在卒中后任何时间点均不相关，但在栓塞后4小时个体血浆BDNF水平与卒中严重程度之间观察到正相关（r=0.67）。 循环中的BDNF水平并不能反映中风后脑中BDNF的水平，严重的中风与非常急性期的高血浆BDNF相关。
Whereas brain-derived neurotrophic factor (BDNF) levels are measured in the brain in animal models of stroke, neurotrophin levels in stroke patients are measured in plasma or serum samples. The present study was designed to investigate the meaning of circulating BDNF levels in stroke patients. Unilateral ischemic stroke was induced in rats by the injection of various numbers of microspheres into the carotid circulation in order to mimic the different degrees of stroke severity observed in stroke patients. Blood was serially collected from the jugular vein before and after (4 h, 24 h and 8 d) embolization and the whole brains were collected at 4, 24 h and 8 d post-embolization. Rats were then selected from their degree of embolization, so that the distribution of stroke severity in the rats at the different time points was large but similar. Using ELISA tests, BDNF levels were measured in plasma, serum and brain of selected rats. Whereas plasma and serum BDNF levels were not changed by stroke, stroke induced an increase in brain BDNF levels at 4 h and 24 h post-embolization, which was not correlated with stroke severity. Individual plasma BDNF levels did not correlate with brain levels at any time point after stroke but a positive correlation (r = 0.67) was observed between individual plasma BDNF levels and stroke severity at 4 h post-embolization. Circulating BDNF levels do not mirror brain BDNF levels after stroke, and severe stroke is associated with high plasma BDNF in the very acute stage.