Global identification of genes associated with xylan biosynthesis in cotton fiber
Global identification of genes associated with xylan biosynthesis in cotton fiber
复制标题
棉纤维中木聚糖生物合成相关基因的全局鉴定
DOI：
10.1186/s42397-020-00063-3
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发表时间：
2020-01-01
影响因子：
2.6
通讯作者：
Xu Wenliang
中科院分区：
文献类型：
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作者：
Chen Feng;Guo Yanjun;Xu Wenliang
背景：成熟棉纤维的次生细胞壁主要由纤维素（约90%）和少量的木聚糖和木质素组成。目前对棉纤维木聚糖的生物合成知之甚少。结果：通过系统发育分析、表达谱分析和共表达分析，鉴定出5个IRX9基因、5个IRX10基因、1个IRX14基因、6个IRX15基因、2个FRA8基因、1个PARVUS基因、8个GUX基因、4个GXM基因、2个RWA基因、2个AXY9基因、13个TBL基因。此外，我们还鉴定了2个GT61成员、2个GT47成员和2个DUF579家族成员，这些成员在拟南芥中的同源物未被功能表征。这55个基因被认为是最有可能参与纤维木聚糖生物合成的基因。进一步的互补分析表明，1个IRX10样基因和2个FRA8相关基因能够在各自的拟南芥突变体中部分恢复木聚糖缺乏所带来的不规则木质部表型。我们得出结论，这些基因是与GX生物合成有关的各自基因的功能同源物。结论：本文所获得的55个棉花基因不仅为揭示棉纤维中木聚糖的生物合成提供了坚实的基础，而且为今后利用生物技术手段进行纤维改良的研究提供了潜在的遗传资源。
Background: Mature cotton fiber secondary cell wall comprises largely of cellulose (> 90%) and small amounts of xylan and lignin. Little is known about the cotton fiber xylan biosynthesis by far.Results: To comprehensively survey xylan biosynthetic genes in cotton fiber, we identified five IRX9, five IRX10, one IRX14, six IRX15, two FRA8, one PARVUS, eight GUX, four GXM, two RWA, two AXY9, 13 TBL genes by using phylogenetic analysis coupled with expression profile analysis and co-expression analyses. In addition, we also identified two GT61 members, two GT47 members, and two DUF579 family members whose homologs in Arabidopsis were not functionally characterized. These 55 genes were regarded as the most probable genes to be involved in fiber xylan biosynthesis. Further complementation analysis indicated that one IRX10 like and two FRA8 related genes were able to partially recover the irregular xylem phenotype conferred by the xylan deficiency in their respective Arabidopsis mutant. We conclude that these genes are functional orthologs of respective genes that are implicated in GX biosynthesis.Conclusion: The list of 55 cotton genes presented here provides not only a solid basis to uncover the biosynthesis of xylan in cotton fiber, but also a genetic resource potentially useful for future studies aiming at fiber improvement via biotechnological approaches.