Quantitative genotyping by amplifying the polymorphic sequences of Pre-Melanosomal Protein (PMEL17) gene using real-time polymerase chain reaction in chickens.
Quantitative genotyping by amplifying the polymorphic sequences of Pre-Melanosomal Protein (PMEL17) gene using real-time polymerase chain reaction in chickens.
复制标题
通过使用实时聚合酶链式反应扩增鸡前黑素体蛋白 (PMEL17) 基因的多态性序列来进行定量基因分型。
DOI：
10.1080/00071660802298310
复制
发表时间：
2008
影响因子：
2
通讯作者：
Harumi T and Naito M
中科院分区：
文献类型：
--
作者：
Minematsu T;Harumi T and Naito M
1.建立了嵌合鸡的实时荧光定量PCR基因分型体系; 2.对白色来航鸡、Barred Plymouth Rock鸡和罗得岛红鸡的羽色主效基因PMEL 17基因第7和第11外显子进行了多态性分析; 3.采用TaqMan探针技术成功地对嵌合鸡的基因进行了定量分型。
1. This study was conducted to develop a quantitative genotyping system of chimaeric chickens by real-time PCR.2. The polymorphisms in exons 7 and 11 ofPMEL17gene, which is one of the major genes affecting plumage colour, were identified from White Leghorn, Barred Plymouth Rock and Rhode Island Red chickens.3. Quantitative genotyping was successfully performed by real-time PCR using polymorphic sequence-specific TaqMan Probes.4. This methodology can support future research of germline chimaeric chickens as well as the application of germ cell transfer technique.