In vivo genotoxicity evaluation of atrazine and atrazine-based herbicide on fish Carassius auratus using the micronucleus test and the comet assay
In vivo genotoxicity evaluation of atrazine and atrazine-based herbicide on fish Carassius auratus using the micronucleus test and the comet assay
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DOI：
10.1016/j.fct.2011.03.038
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发表时间：
2011-06-01
影响因子：
4.3
通讯作者：
Cavas, Tolga
中科院分区：
文献类型：
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作者：
Cavas, Tolga
阿特拉津是一种选择性三嗪类除草剂，主要用于防治玉米、高粱、甘蔗、菠萝等作物以及针叶树重新造林种植田的阔叶杂草和禾本科杂草。研究表明，过去二十年来，莠去津是农业溪流和河流中最常检测到的农药之一。尽管莠去津的毒性众所周知，但关于莠去津对水生生物的遗传毒性作用的数据却相当缺乏。因此，在本研究中，我们旨在使用外周血红细胞中的微核测试和彗星测定来评估莠去津和基于莠去津的除草剂（Gesaprim (R)）对模型鱼类鲫鱼（Carassius auratus L, 1758）（双鱼座：鲤科）的基因毒性作用。将鱼暴露于5、10和15μg/L莠去津及其商业制剂中2、4和6天。单剂量5mg/L的甲磺酸乙酯(EMS)用作阳性对照。我们的结果表明，在接触莠去津商业制剂后，金鲫红细胞中微核和 DNA 链断裂的频率显着增加，从而证明了这种农药对鱼类的潜在遗传毒性。 (C) 2011 Elsevier Ltd. 保留所有权利。
Atrazine is a selective triazine herbicide used to control broadleaf and grassy weeds mainly in corn, sorghum, sugarcane, pineapple, and other crops, and in conifer reforestation planting fields. It has been showed that atrazine is one of the most frequently detected pesticides in agricultural streams and rivers, over the past two decades. Although the toxic properties of atrazine are well known, the data on the genotoxic effects of atrazine on aquatic organisms are rather scarce. Thus, in the present study we aimed to evaluate the genotoxic effects of atrazine and an atrazine-based herbicide (Gesaprim (R)) on a model fish species Carassius auratus L, 1758, (Pisces: Cyprinidae) using the micronucleus test and the comet assay in peripheral blood erythrocytes. Fish were exposed to 5, 10 and 15 mu g/L atrazine and to its commercial formulation for 2,4 and 6 days. Ethyl methane sulfonate (EMS) at a single dose of 5 mg/L was used as positive control. Our results revealed significant increases in the frequencies of micronuclei and DNA strand breaks in erythrocytes of C. auratus, following exposure to commercial formulation of atrazine and thus demonstrated the genotoxic potential of this pesticide on fish. (C) 2011 Elsevier Ltd. All rights reserved.