Allogenic peripheral blood derived mesenchymal stem cells (MSCs) enhance bone regeneration in rabbit ulna critical-sized bone defect model
Allogenic peripheral blood derived mesenchymal stem cells (MSCs) enhance bone regeneration in rabbit ulna critical-sized bone defect model
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DOI：
10.1002/jor.20119
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发表时间：
2006-04-01
影响因子：
2.8
通讯作者：
Li, G
中科院分区：
文献类型：
--
作者：
Wan, C;He, QL;Li, G
间充质干细胞（MSC）已被证明存在于几种哺乳动物的外周血（PB）中，包括人、豚鼠、小鼠、大鼠和兔。PB来源的MSCs（PBMSCs）是否能促进大面积骨缺损的再生尚未见报道。在这项研究中，兔骨髓间充质干细胞获得的PB和骨髓（BM）来源的单核细胞（MNCs）培养物。PBMSCs的数量相对较低，殖民地形成效率（CFE）范围为1.2 - 13/百万MNC。在特定的诱导条件下，PBMSCs可分化为成骨细胞、软骨细胞和脂肪细胞，表现出与BMMSCs相似的多向分化能力。在12只6月龄新西兰白色兔的尺骨中制造双侧20 mm临界尺寸的骨缺损。用同种异体PBMSCs/Skelite（多孔磷酸钙可吸收替代物）、BMMSCs/Skelite、PBMNCs/ Skelite、单独Skelite处理缺损，并保持空白12周。通过连续X线摄影、外周定量计算机断层扫描（pQCT）和组织学检查评价骨再生。PBMSCs/Skelite和BMMSCs/ Skelite治疗组的X线评分和pQCT总骨密度分别显著大于PBMNC/Skelite和单独Skelite组（p < 0.05）。组织学上，在PBMSC/ Skelite和BMMSC/Skelite处理组中新形成的骨是明显的。结果表明，兔PBMSCs具有与BMMSCs相当的多向分化潜能，同种异体PBMSCs接种于多孔磷酸钙可吸收替代物上，可促进兔尺骨临界尺寸骨缺损模型的骨再生，提示同种异体PBMSCs可能成为骨再生和组织工程中新的循环成骨干细胞来源。(c)2006骨科研究学会。
Mesenchymal stem cells (MSCs) were demonstrated to exist within peripheral blood (PB) of several mammalian species including human, guinea pig, mice, rat, and rabbit. Whether or not the PB derived MSCs (PBMSCs) could enhance the regeneration of large bone defects have not been reported. In this study, rabbit MSCs were obtained from mononuclear cells (MNCs) cultures of both the PB and bone marrow (BM) origin. The number of PBMSCs was relatively lower, with the colony forming efficiency (CFE) ranging from 1.2 to 13 per million MNCs. Under specific inductive conditions, PBMSCs differentiated into osteoblasts, chondrocytes, and adipocytes, showing multidifferentiation ability similar to BMMSCs. Bilateral 20 mm critical-sized bone defects were created in the ulnae of 12 6-month-old New Zealand white rabbits. The defects were treated with allogenic PBMSCs/Skelite (porous calcium phosphate resorbable substitute), BMMSCs/Skelite, PBMNCs/ Skelite, Skelite alone, and left empty for 12 weeks. Bone regeneration was evaluated by serial radiography, peripheral quantitative computed tomography (pQCT), and histological examinations. The X-ray scores and the pQCT total bone mineral density in the PBMSCs/Skelite and BMMSCs/ Skelite treated groups were significantly greater than those of the PBMNCs/Skelite and Skelite alone groups (p < 0.05), respectively. Histologically, newly formed bone was evident in the PBMSCs/ Skelite and BMMSCs/Skelite treated groups. The findings demonstrated that the rabbit PBMSCs possessed multidifferentiation potential comparable with BMMSCs, allogenic PBMSCs seeded onto porous calcium phosphate resorbable substitutes enhanced bone regeneration in the rabbit ulna critical-sized bone defect model, suggesting allogenic PBMSCs may be a new source of circulating osteogenic stem cells for bone regeneration and tissue engineering. (c) 2006 Orthopaedic Research Society.