Clathrin-Inspired Coating and Stabilization of Liposomes by DNA Self-Assembly
Clathrin-Inspired Coating and Stabilization of Liposomes by DNA Self-Assembly
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DOI：
10.26434/chemrxiv.8859017.v1
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发表时间：
2019-07
期刊：
影响因子：
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通讯作者：
Kevin N. Baumann;L. Piantanida;J. García-Nafría;Diana Sobota;Kislon Voïtchovsky;T. Knowles;Silvia HERNANDEZ AINSA
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文献类型：
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作者：
Kevin N. Baumann;L. Piantanida;J. García-Nafría;Diana Sobota;Kislon Voïtchovsky;T. Knowles;Silvia HERNANDEZ AINSA
蛋白质网格蛋白在生物膜上的自组装促进了生物系统中内吞作用的基本过程，并通过高度有序的结构外观为材料设计提供了灵感来源。通过模拟网格蛋白自组装体的结构以用生物材料包覆脂质体，可以衍生出新类别的混合载体。在这里，我们提出了一种用于制造DNA包被的脂质体的方法，通过疏水锚定和随后生长的DNA网络的脂质体表面上的结构模仿网格蛋白组件。动态光散射（DLS），超电势和低温电子显微镜（cryo-EM）测量独立地证明成功的DNA涂层。用原子力显微镜（AFM）进行的纳米力学测量表明，相对于未涂覆的脂质体，DNA涂层增强了脂质体的机械稳定性。此外，我们提供了通过支点介导的置换反应触发DNA涂层的解体来逆转涂层过程的可能性。我们的研究结果描述了一个简单的，多功能的，可逆的方法涂层和稳定脂质囊泡的交织的DNA网络。该方法具有进一步发展的潜力，对脂质体表面上的定制功能的有序排列和作为混合纳米载体的应用。
The self-assembly of the protein clathrin on biological membranes facilitates essential processes of endocytosis in biological systems and has provided a source of inspiration for materials design by the highly ordered structural appearance. By mimicking the architecture of clathrin self-assemblies to coat liposomes with biomaterials, new classes of hybrid carriers can be derived. Here we present a method for fabricating DNA-coated liposomes by hydrophobically anchoring and subsequently growing a DNA network on the liposome surface which structurally mimics clathrin assemblies. Dynamic light scattering (DLS), ζ-potential and cryo-electron microscopy (cryo-EM) measurements independently demonstrate successful DNA coating. Nanomechanical measurements conducted with atomic force microscopy (AFM) show that the DNA coating enhances the mechanical stability of the liposomes relative to uncoated ones. Furthermore, we provide the possibility to reverse the coating process by triggering the disassembly of the DNA coating through a toehold-mediated displacement reaction. Our results describe a straightforward, versatile, and reversible approach for coating and stabilizing lipid vesicles by an interlaced DNA network. This method has potential for further development towards the ordered arrangement of tailored functionalities on the surfaces of liposomes and for applications as hybrid nanocarrier.