A randomized controlled trial of green tea catechins in protection against ultraviolet radiation-induced cutaneous inflammation.
A randomized controlled trial of green tea catechins in protection against ultraviolet radiation-induced cutaneous inflammation.
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DOI：
10.3945/ajcn.115.107995
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发表时间：
2015-09
期刊：
影响因子：
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通讯作者：
Rhodes LE
中科院分区：
文献类型：
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作者：
Farrar MD;Nicolaou A;Clarke KA;Mason S;Massey KA;Dew TP;Watson RE;Williamson G;Rhodes LE
背景：对阳光中紫外线辐射(UVR)的健康危害进行安全的系统性防护是可取的。绿茶在全球范围内被消费，据报道具有抗炎特性，这可能是通过影响环氧合酶和脂氧合酶途径来调节的。最近的数据表明，绿茶儿茶素(GTCS)可以减少急性UVR效应，但检验其光保护潜力的人体试验很少。目的：我们进行了一项双盲、随机、安慰剂对照试验，以检验GTCS是否对UVR诱导的炎症的临床、组织学和生化指标具有保护作用。设计：健康成人(年龄18-65岁，照片类型I-II)随机分为1350 mg绿茶提取物(540 Mg GTC)和50 mg维生素C或安慰剂，每日2次，共3个月。评估了日光模拟UVR激发后对皮肤红斑、真皮白细胞渗透和促炎二十烷类化合物浓度的影响，并通过测定尿中GTC代谢物表没食子儿茶素葡萄糖醛酸来确定受试者的依从性。结果：志愿者被分配到活动组(n=25)和安慰剂组(n=25)。补充后，试验组和安慰剂组的中位数(IQR)晒伤阈值(最小红斑剂量)分别为28(20-28)mJ/cm2和20(20-28)mJ/cm2(无显著意义)，在10个UVR剂量的几何序列后，测量的红斑指数的AUC分析没有差异。皮肤免疫组织化学显示两组患者中性粒细胞和CD3+T淋巴细胞数量在紫外线照射后均有增加(P<；0.01)，但补充后两组间差异无统计学意义。环氧合酶和脂氧合酶代谢产物前列腺素E_2(血管扩张剂)和12-羟基二十碳四烯酸(化学诱导剂)在UVR后升高(P<；0.05)，补充组之间无差异。结论：口服GTC(1080 mg/d)和维生素C 3个月以上并不能显著减少皮肤红斑、白细胞浸润或对UVR炎症刺激的二十烷基硫酸酯反应。这项试验在Clinicaltrials.gov上注册为NCT01032031。
Background: Safe systemic protection from the health hazards of ultraviolet radiation (UVR) in sunlight is desirable. Green tea is consumed globally and is reported to have anti-inflammatory properties, which may be mediated through the impact on cyclooxygenase and lipoxygenase pathways. Recent data suggest that green tea catechins (GTCs) reduce acute UVR effects, but human trials examining their photoprotective potential are scarce. Objective: We performed a double-blind, randomized, placebo-controlled trial to examine whether GTCs protect against clinical, histologic, and biochemical indicators of UVR-induced inflammation. Design: Healthy adults (aged 18–65 y, phototypes I–II) were randomly allocated to 1350 mg encapsulated green tea extract (540 mg GTC) with 50 mg vitamin C or placebo twice daily for 3 mo. Impact on skin erythema, dermal leukocytic infiltration, and concentrations of proinflammatory eicosanoids was assessed after solar-simulated UVR challenge, and subject compliance was determined through assay of urinary GTC metabolite epigallocatechin glucuronide. Results: Volunteers were assigned to the active (n = 25) or the placebo (n = 25) group. After supplementation, median (IQR) sunburn threshold (minimal erythema dose) was 28 (20–28) and 20 (20–28) mJ/cm2 in the active and placebo groups, respectively (nonsignificant), with no difference in AUC analysis for measured erythema index after a geometric series of 10 UVR doses. Skin immunohistochemistry showed increased neutrophil and CD3+ T-lymphocyte numbers post-UVR in both groups (P < 0.01) with no statistically significant differences between groups after supplementation. Cyclooxygenase and lipoxygenase metabolites prostaglandin E2 (vasodilator) and 12-hydroxyeicosatetraenoicacid (chemoattractant), respectively, increased after UVR (P < 0.05), with no differences between supplementation groups. Conclusion: Oral GTC (1080 mg/d) with vitamin C over 3 mo did not significantly reduce skin erythema, leukocyte infiltration, or eicosanoid response to UVR inflammatory challenge. This trial was registered at clinicaltrials.gov as NCT01032031.