Chronic exposure of 2,2′,4,4′-tetrabromodiphenyl ether (PBDE-47) alters locomotion behavior in juvenile zebrafish (Danio rerio)
Chronic exposure of 2,2′,4,4′-tetrabromodiphenyl ether (PBDE-47) alters locomotion behavior in juvenile zebrafish (Danio rerio)
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DOI：
10.1016/j.aquatox.2010.03.012
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发表时间：
2010-07-15
影响因子：
4.5
通讯作者：
Chen, Te-Hao
中科院分区：
文献类型：
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作者：
Chou, Chun-Ting;Hsiao, Yu-Chen;Chen, Te-Hao
在本研究中，我们使用斑马鱼（Danio rerio）作为一个模型，以解决可能的影响，长期暴露的多溴联苯醚（PBDE）阻燃剂对鱼类的运动行为，身体大小和性腺发育。从孵化后21天（dph）至90 dph，用PBDE-47（对照、溶剂对照、低、中和高剂量组）喂食斑马鱼。鱼的运动参数，包括最大游泳速度，总距离移动，和百分比时间活跃，使用基于视频的动物运动分析系统进行了评估。在接触结束时，对所有鱼实施安乐死，以测量长度和重量，然后对整条鱼进行组织学分析或组织PBDE-47测量。五组之间的存活率、体型和性腺组织学相似。然而，总游泳距离和活动时间百分比与组织中PBDE-47浓度呈负相关，并且在高剂量组中显著较低。在接触过PBDE-47的鱼类体内，组织中的PBDE-47含量与以往实地研究报告的含量相当。总之，这项研究表明，斑马鱼在发育过程中接触到与生态相关的多溴二苯醚-47会改变其运动行为，但不会影响其体型或性腺发育。(C)2010爱思唯尔有限公司版权所有。
In the present study, we used zebrafish (Danio rerio) as a model to address possible effects of chronic exposure of polybrominated diphenyl ether (PBDE) flame retardants on locomotion behavior, body size, and gonad development in fish. Zebrafish were fed food dosed with PBDE-47 (control, solvent control, low, medium, and high dose groups) from 21 days post hatch (dph) to 90 dph. Fish locomotion parameters, including maximum swimming speed, total distance moved, and percent time active, were assessed using a video-based animal movement analysis system. At the end of the exposure, all fish were euthanized for length and weight measurement, and then subjected to either whole fish histological analysis or tissue PBDE-47 measurement. Survival, body size, and gonad histology were similar between the five groups. However, both total swimming distance and percent time active were negatively correlated with tissue PBDE-47 concentration and were significantly lower in the high dose group. Tissue levels of PBDE-47 in the exposed fish were comparable to that reported in previous field studies. In summary, this study showed that developmental exposure of PBDE-47 at an ecologically relevant level altered locomotion behavior without affecting body size or gonad development of zebrafish. (C) 2010 Elsevier B.V. All rights reserved.