Identification of genes expressed in the tobacco shoot apex during the floral transition.
Identification of genes expressed in the tobacco shoot apex during the floral transition.
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花转变期间烟草芽尖表达基因的鉴定。
DOI：
10.1105/tpc.2.10.963
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Meeks-Wagner,DR
中科院分区：
文献类型：
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作者：
Kelly,AJ;Zagotta,MT;White,RA;Chang,C;Meeks-Wagner,DR
高等植物的顶端含有未分化的分生组织细胞，这些细胞是胚胎后器官的起源。从营养生长到生殖生长的转变导致顶端分生组织致力于花器官的形成。为了确定启动花发育的分子信号，我们分离了烟草从营养生长到花生长过渡过程中在顶端转录活跃的基因。顶端的小尺寸使我们使用了聚合酶链式反应的顶端。这种方法能够分离到本文中描述的顶端特异的和花端特异的cDNA克隆。其中一个克隆A3在观察到的所有发育阶段的顶芽中检测到同等水平的转录本。第二个克隆FA2检测到一个独特的转录本，该转录本在花芽顶端开花的过程中含量增加，并在发育中的花瓣、雄蕊和雌蕊中显示出高水平的表达。
The shoot apex of higher plants contains undifferentiated meristematic cells that serve as the origin of post-embryonic organs. The transition from vegetative to reproductive growth results in the commitment of the apical meristem to floral organ formation. To identify the molecular signals that initiate floral development, we have pursued the isolation of genes that are transcriptionally active in the shoot apex of tobacco during the transition from vegetative to floral growth. The small size of the apex led us to utilize polymerase chain reaction shoot apices. This approach enabled the isolation of the apex-specific and floral apex-specific cDNA clones described in this paper. One clone, A3, detected an equivalent level of transcript in the shoot apex during all developmental stages observed. The second clone, FA2, detected a unique transcript that increased in abundance in the shoot apex during the transition to flowering and showed high levels of expression in developing petals, stamens, and pistils.