Plant extracts as natural amoebicidal agents
Plant extracts as natural amoebicidal agents
复制标题
DOI：
10.1007/s00436-008-1277-9
复制
发表时间：
2009-02-01
影响因子：
2
通讯作者：
Thiem, Barbara
中科院分区：
文献类型：
--
作者：
Derda, Monika;Hadas, Edward;Thiem, Barbara
棘阿米巴属菌株是导致慢性肉芽肿性阿米巴脑炎、角膜炎、肺炎以及其他器官炎症的一个因素。这些疾病的治疗非常困难，而且并不总是有效。这些感染大多数是致命的。本研究的目的是研究黄连、葛根、金牛花和禾本科花等植物提取物的杀阿米巴或抑制阿米巴活性。为了分离药物活性物质，我们使用了植物的地上部分，连同花、根和叶。结果表明，在0.01 ~ 0.05 mg提取物/mL的浓度范围内，紫花葡萄、野檀和山黧豆的提取物具有体外化疗作用，即山黧豆的鞣花酸表达浓度为0.350 μ g/mL，山黧豆的葛根素表达浓度为0.053 μ g/mL。治疗指数值为3.5 ~ 20。体内实验结果表明，在使用提取物治疗后，感染棘阿米巴原虫的动物存活时间延长（延长2.5-3倍）。经确定，植物提取物可用于外部和内部的联合治疗棘阿米巴病的情况下。被测试的提取物对动物没有毒性。
Strains of Acanthamoeba sp. constitute a factor contributing to the occurrence of chronic granulomatous amoebic encephalitis, keratitis, pneumonia, as well as inflammations of other organs. Treatment of these diseases is very difficult and not always effective. A majority of these infections have been fatal. The aim of our study was to examine the amoebicidal or amoebistatic activity of plant extracts from Rubus chamaemorus, Pueraria lobata, Solidago virgaurea and Solidago graminifolia. For the purpose of isolation of pharmacologically active substances, we used the aboveground parts of plants, together with flowers, roots and leaves. It was established that extracts from S. virgauera, P. lobata and R. chamaemorus displayed chemotherapeutic properties in vitro in concentrations of approximately 0.01-0.05 mg extract/mL, i.e. in concentrations of 0.350 mu g/mL expressed in ellagic acid for R. chamaemorus and 0.053 mu g/mL expressed in puerarin for P. lobata. Therapeutic index values is 3.5-20. As a result of in vivo experiments, it was found out that, following therapy using the extracts, animals infected with Acanthamoeba sp. survived for an extended period (2.5-3 times longer). It was determined that plant extracts may be used both externally and internally in the case of a combined therapy for acanthamoebiasis. The tested extracts are not toxic for animals.