Low-temperature Degradation Mechanism Analysis of Petroleum Hydrocarbon-degrading Antarctic Psychrophilic Strains
Low-temperature Degradation Mechanism Analysis of Petroleum Hydrocarbon-degrading Antarctic Psychrophilic Strains
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通讯作者：
Yi-bin Wang;Fang-ming Liu;Liang Qiang;Bi-juan He;Jin-lai Miao
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作者：
Yi-bin Wang;Fang-ming Liu;Liang Qiang;Bi-juan He;Jin-lai Miao
激光镊子拉曼光谱（LTRS）可以帮助观察和研究自然状态下的单个细胞或细胞器相对较长的时间。本研究利用LTRS和GC-MS技术对分离自南极海洋的嗜冷石油烃降解菌的生理代谢进行了研究。GC-MS分析和降解产物的拉曼光谱分析表明，Planococcus sp. NJ 41和Shewanella sp. NJ 49在低温（0°C ~ 10 ° C）条件下对柴油、正十六烷、多环芳烃等石油烃具有较高的降解效率。GC-MS分析表明，长直链烃被分解为短直链烃。拉曼光谱表明，在Planococcus sp. NJ 41和Shewanella sp. NJ 49的生长和降解过程中，产生的蛋白质和碳水化合物多于脂类。最后，在1512 cm-1至1514 cm-1处的拉曼强度代表了β -胡萝卜素，它参与调节南极细菌的膜流动性，使其能够适应南极的极端低温环境。
Laser tweezers Raman spectroscopy (LTRS) can help with observing and studying individual cells or organelles in a natural state for a relatively long period. In this study, LTRS and GC-MS were used to report physiological metabolism of active psychrophilic petroleum hydrocarbon-degrading strains isolated from the Antarctic Ocean. Based on GC-MS analysis and analysis of the Raman spectrum of degradation productions, the results showed Planococcus sp.NJ41 and Shewanella sp.NJ49 degraded diesel, n-hexadecane, polycyclic aromatic hydrocarbons, and other petroleum hydrocarbons with high efficiency at low temperature (0°C -10°C). GC-MS showed the long straight-chain hydrocarbons were decomposed into short straight-chain hydrocarbons. The Raman spectrum showed there were more proteins and carbohydrate than lipids were produced during the Planococcus sp.NJ41 and Shewanella sp.NJ49 growth and degradation. Finally, the Raman intensity at 1512 cm -1 to 1514cm -1 represented βββββ -carotene that was involved in the regulation of membrane fluidity in Antarctic bacteria, allowing it to adapt to the extreme low-temperature environment of the Antarctic.