Seven members of the (1→3)-β-glucanase gene family in barley (Hordeum vulgare) are clustered on the long arm of chromosome 3 (3HL)
Seven members of the (1→3)-β-glucanase gene family in barley (Hordeum vulgare) are clustered on the long arm of chromosome 3 (3HL)
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DOI：
10.1007/bf00221889
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发表时间：
1996-05
影响因子：
5.4
通讯作者：
C. Li;P. Langridge;R. Lance;P. Xu;G. Fincher
中科院分区：
文献类型：
--
作者：
C. Li;P. Langridge;R. Lance;P. Xu;G. Fincher
利用3个双单倍体群体和7个小麦-大麦异附加系，将（1 → 3）-β-葡聚糖葡聚糖水解酶（EC www.example.com）基因家族的成员定位在大麦基因组上。针对目前可获得cDNA或基因组克隆的7个大麦（1 → 3）-β-葡聚糖酶基因产生特异性探针或聚合酶链反应（PCR）引物。这7个基因均位于3号染色体长臂上（3HL），编码同工酶GI、GII、GIII、GIV、GV和GVII的基因（ABG2）聚集在长度小于20 cM的区域内。该区域的近端侧接RFLP标记MWG2099，远端侧接大麦黄花叶病毒（BYMV）抗性基因m4。编码同工酶GVI的基因位于该簇外约50 cM处，朝向着丝粒。除了编码同工酶GIV的基因之外，所有（1 → 3）-β-葡聚糖酶基因在大麦基因组上均以单拷贝表示。在Southern印迹分析中，GIV同工酶基因探针与4条DNA带杂交，其中只有一条能被整合到共有连锁图谱中。
Members of the (1→3)-β-glucan glucanohydrolase (EC 3.2.1.39) gene family have been mapped on the barley genome using three doubled haploid populations and seven wheat-barley addition lines. Specific probes or polymerase chain reaction (PCR) primers were generated for the seven barley (1→3)-β-glucanase genes for which cDNA or genomic clones are currently available. The seven genes are all located on the long arm of chromosome 3 (3HL), and genes encoding isoenzymes GI, GII, GIII, GIV, GV and GVII (ABG2) are clustered in a region less than 20 cM in length. The region is flanked by the RFLP marker MWG2099 on the proximal side and the Barley Yellow Mosaic Virus (BYMV) resistance geneym4at the distal end. The gene encoding isoenzyme GVI lies approximately 50 cM outside this cluster, towards the centromere. With the exception of the gene encoding isoenzyme GIV, all of the (1→3)-β-glucanase genes are represented by single copies on the barley genome. The probe for the isoenzyme GIV gene hybridized with four DNA bands during Southern blot analysis, only one of which could be incorporated into the consensus linkage map.