IDENTIFICATION OF THE MOLECULAR-SPECIES OF LYSOPHOSPHATIDIC ACID PRODUCED WHEN PLATELETS ARE STIMULATED BY THROMBIN
IDENTIFICATION OF THE MOLECULAR-SPECIES OF LYSOPHOSPHATIDIC ACID PRODUCED WHEN PLATELETS ARE STIMULATED BY THROMBIN
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DOI：
10.1016/0005-2760(89)90112-4
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发表时间：
1989-02-20
期刊：
影响因子：
--
通讯作者：
ROBINSON, P
中科院分区：
文献类型：
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作者：
GERRARD, JM;ROBINSON, P
当3U凝血酶/109血小板搅拌时，产生大量棕榈酰化溶血磷脂酸(2.17 ng/109血小板)、硬脂酰溶血磷脂酸(2.11 ng/109血小板)和花生四烯酰溶血磷脂酸(1.06 ng/109血小板)。当用磷脂酶A2抑制剂U10029A的100µm处理血小板时，凝血酶刺激的硬脂酰溶血磷脂酸的产生显著减少(降至0.16 ng/109血小板)，而花生四烯酰溶血磷脂酸的产生不变。U10029A同时增加了凝血酶刺激的含有硬脂酰基的磷脂酸种类(主要是硬脂酰基磷脂酸)的产生，从5.99 ng/109血小板增加到9.71 ng/109血小板。这一结果与硬脂酰化溶血磷脂酸是通过磷脂酶A2降解磷脂酸而在血小板中产生的概念一致。
Platelets, when stirred with 3 U thrombin/109 platelets, produced significant quantities of palmitoyllysophosphatidic acid (2.17 ng/109 platelets), stearoyllysophosphatidic acid (2.11 ng/109 platelets), and arachidonoyllysophosphatidic acid (1.06 ng/109 platelets). When platelets were pretreated with 100 .mu.M of the phospholipase A2 inhibitor U10029A, there was a significant decrease in thrombin-stimulated production of stearoyllysophosphatidic acid (to 0.16 ng/109 platelets), while arachidonoyllysophosphatidic acid production was unchanged. U10029A concomitantly increased thrombin-stimulated production of stearoyl-containing phosphatidic acid species (primarily stearoylarachildonoylphosphatidic acid) from 5.99 to 9.71 ng/109 platelets. The results are consistant with the concept that stearoyllysophosphatidic acid production in platelets occurs via phospholipase A2 degradation of phosphatidic acid.