Measuring spatial variation in natural selection using randomly‐sown seeds of Arabidopsis thaliana
Measuring spatial variation in natural selection using randomly‐sown seeds of Arabidopsis thaliana
复制标题
使用随机播种的拟南芥种子测量自然选择的空间变化
DOI：
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发表时间：
1996
期刊：
影响因子：
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通讯作者：
C. Bennington
中科院分区：
文献类型：
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作者：
D. Stratton;C. Bennington
我们描述了一个简单的方法来测量空间变化的健身使用随机播种的种子。给定种子的随机初始分布，基因型最终分布的任何空间变异都必然是自然选择中空间变异的结果。使用修改的连接计数统计量来测试空间随机性的结果，基于两个随机选择的相隔给定距离的植物携带相同遗传标记的概率。蒙特卡罗模拟表明，对于随机播种的种子设计，联合计数检验比方差分析更能检测适应度的空间变异。的权力是相对独立的基因型的数量或采样的微位点的数量。该方法在田间使用拟南芥的三个标准品系进行了测试。在生命周期结束时，相邻的植物显着更有可能是同一行比随机选择的对和空间依赖性扩展到近50厘米的距离。像这样的简单实验可能对“比较生态遗传学”特别有用，可以系统地研究许多物种或栖息地的自然选择。
We describe a simple method of measuring spatial variation in fitness using randomly sown seeds. Given a random initial distribution of seeds, any spatial variation in the final distribution of genotypes must be the result of spatial variation in natural selection. Departures from spatial randomness are tested using a modification of join‐count statistics, based on the probability that two randomly chosen plants separated by a given distance carry the same genetic marker. Monte Carlo simulations showed that for the randomly sown seeds design the join‐count test had higher power to detect spatial variation in fitness than analysis of variance. The power was relatively independent of the number of genotypes used or the number of microsites sampled. The method was tested in the field using three standard lines of Arabidopsis thaliana. At the end of the life cycle, adjacent plants were significantly more likely to be the same line than randomly chosen pairs and the spatial dependence extended to distances of nearly 50 cm. Simple experiments like this may be especially useful for “comparative ecological genetics”, allowing systematic studies of natural selection among many species or habitats.