Polymorphic microsatellites developed by cross-species amplifications in common pheasant breeds
Polymorphic microsatellites developed by cross-species amplifications in common pheasant breeds
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DOI：
10.1046/j.1365-2052.2001.00767.x
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发表时间：
2001-08-01
期刊：
影响因子：
2.4
通讯作者：
Fulgheri, FD
中科院分区：
文献类型：
--
作者：
Baratti, M;Alberti, A;Fulgheri, FD
对两个常见雉鸡品种的遗传变异进行了分析。1758）的方法进行微卫星位点的跨物种扩增：154只鸡，原鸡和32只火鸡，Meleagris gallopavo。测试引物以扩增野鸡DNA。36个引物（25个鸡特异性引物和11个火鸡特异性引物）扩增野鸡DNA。15个标记产生了特定的产物，并进行了多态性测试。其中8个（55%）是多态性的，平均多态性的两个等位基因每个位点。对特异性聚合酶链反应（PCR）产物进行测序，在15个标记中的11个中发现了重复序列，尽管只有两个位点显示相同的重复序列，并且可以与鸡的同源。
Genetic variability was analysed in two common breeds of pheasant (Phasianus colchicus L. 1758) by means of cross-species amplifications of microsatellite loci: 154 chicken, Gallus gallus and 32 turkey, Meleagris gallopavo. primers were tested for amplification of pheasant DNA. Thirty-six primers (25 specific for chicken and 11 for turkey) amplified pheasant DNA. Fifteen markers yielded specific products and were tested for polymorphism. Eight of them (55%) were polymorphic, with an average polymorphism of two alleles per locus. Specific polymerase chain reaction (PCR) products were sequenced; repeats were found in 11 of the 15 markers, although only two loci showed the same repeat and could be homologous to chicken ones.