INTRAGINGIVAL OCCURRENCE OF ACTINOBACILLUS-ACTINOMYCETEMCOMITANS AND BACTEROIDES-GINGIVALIS IN ACTIVE DESTRUCTIVE PERIODONTAL LESIONS
INTRAGINGIVAL OCCURRENCE OF ACTINOBACILLUS-ACTINOMYCETEMCOMITANS AND BACTEROIDES-GINGIVALIS IN ACTIVE DESTRUCTIVE PERIODONTAL LESIONS
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DOI：
10.1902/jop.1988.59.4.259
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发表时间：
1988-04-01
影响因子：
4.3
通讯作者：
CAMARGO, P
中科院分区：
文献类型：
--
作者：
SAGLIE, FR;MARFANY, A;CAMARGO, P
共6个活动和6个非活动的网站，从6个未经治疗的牙周炎患者进行了检查，牙龈内存在放线杆菌放线菌伴放线杆菌和牙龈拟杆菌。活动性破坏性牙周病的判定采用“耐受度法”。“免疫过氧化物酶法用于鉴定活动和非活动牙周部位的龈内微生物。用过氧化物酶标记的抗A.放线菌共生菌和B.牙龈炎B。与非活性位点相比，发现活性位点的结缔组织中牙龈炎显著升高。在被A侵袭的结缔组织中，活动部位和非活动部位之间存在统计学显著的边界线差异。放线菌属我们的发现加上A.放线菌共生菌和B. gingivalis支持这些细菌是牙周炎中重要的侵入性病原体的概念。
A total of six active and six nonactive sites from six untreated periodontitis patients were examined for intragingival presence of Actinobacillus actinomycetemcomitans and Bacteroides gingivalis. The active destructive periodontal disease was determined by the "tolerance method." The method of immuoperoxidase was used in the identification of intragingival microoganisms in active and nonactive periodontal sites. Light microscopic sections of gingival tissues consecutive to those with gram stain, revealing presence of bacteria (substantiated by electron microscopy), were stained with peroxidase-labeled antibodies against A. actinomycetemocomitans and B. gingivalis. B. gingivalis was found to be significantly elevated in the connective tissue of active sites when compared to nonactive sites. A statistically significant border-line difference was found between active and nonactive sites in the connective tissue invaded by A. actinomycetemecomitans. Our findings plus the well established periodontopathic potential of A. actinomycetemcomitans and B. gingivalis support the concept that these bacteria are important invasive pathogenic agents in periodontitis.