NET CALCIUM EXCHANGE IN ADULT-RAT VENTRICULAR MYOCYTES - AN ASSESSMENT OF MITOCHONDRIAL CALCIUM ACCUMULATING CAPACITY
NET CALCIUM EXCHANGE IN ADULT-RAT VENTRICULAR MYOCYTES - AN ASSESSMENT OF MITOCHONDRIAL CALCIUM ACCUMULATING CAPACITY
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DOI：
10.1098/rspb.1984.0091
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发表时间：
1984-01-01
期刊：
影响因子：
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通讯作者：
WARD, JPT
中科院分区：
文献类型：
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作者：
FRY, CH;POWELL, T;WARD, JPT
在洋地黄苷处理后的心肌细胞悬液中测量了净钙交换。这种交换被认为是通过线粒体膜进行的，可以通过增加或阻断线粒体电子传递的药物来刺激或抑制。钙的吸收对悬浮液pCa有很强的依赖性，但在1 μm以下不明显（pCa 6.0）。认为净钙交换是两个过程的平衡，这两个过程等价于pca 6.0。通过测量线粒体钙摄取的特定活性，可以计算出心肌线粒体在突然升高后对肌浆钙的影响速度。这表明，细胞器可能部分影响松弛，特别是在收缩的高峰期。
Net calcium exchange has been measured in a suspension of cardiac myocytes after treatment with digitonin. The exchange is believed to be across the mitochondrial membranes and can be stimulated or inhibited by agents augmenting or blocking mitochondrial electron transport. The uptake of calcium shows a strong dependence on suspensionpCa but is not evident below 1 μm (pCa 6.0). It is suggested that the net calcium exchange is a balance of the two processes which are equivalent atpCa 6.0. The measurement of mitochondrial specific activity for calcium uptake allows a calculation of the rapidity with which the cardiac mitochondria would affect sarcoplasmic calcium after a sudden rise. It is suggested that the organelle could partly affect relaxation especially at the peak of contraction.