Applying Raman Microspectroscopy to Evaluate the Effects of Nutrient Cations on Alkane Bioavailability to Acinetobacter baylyi ADP1
Applying Raman Microspectroscopy to Evaluate the Effects of Nutrient Cations on Alkane Bioavailability to Acinetobacter baylyi ADP1
复制标题
DOI：
10.1021/acs.est.0c04944
复制
发表时间：
2020-12-15
影响因子：
11.4
通讯作者：
Martin, Francis L.
中科院分区：
文献类型：
--
作者：
Li, Hanbing;Zhang, Dayi;Martin, Francis L.
石油碳氢化合物污染对生态系统造成广泛破坏。在石油污染场地，烷烃是主要成分；许多本土细菌可以接触和/或降解烷烃。然而，它们这样做的能力受到土壤外部特性（包括养分阳离子）的影响。本研究使用拉曼显微光谱来研究营养物阳离子如何影响烷烃对贝氏不动杆菌 ADP1（一种已知的降解剂）的生物利用度。用 10 mM Na、K、Mg 和 Ca 处理后，A. baylyi 暴露于七种正烷烃（癸烷、十二烷、十四烷、十六烷、十九烷、二十烷和二十四烷）和一种烷烃混合物（矿物油）。拉曼光谱分析表明烷烃的生物利用度随碳链长度的变化而变化，并且额外的阳离子改变了细菌对正烷烃的反应。钠显着增加了细菌对癸烷和十二烷的亲和力，钾和镁增强了十四烷和十六烷的生物利用度。相比之下，所有烷烃的细菌反应均被 Ca 抑制。在矿物油暴露中也观察到类似的结果。我们的研究采用拉曼光谱分析来深入了解营养物阳离子如何影响烷烃的生物利用度，表明营养物阳离子在影响碳氢化合物的有害影响方面可以发挥关键作用，并且可以进行优化以增强生物修复策略。
Contamination with petroleum hydrocarbons causes extensive damage to ecological systems. On oil-contaminated sites, alkanes are major components; many indigenous bacteria can access and/or degrade alkanes. However, their ability to do so is affected by external properties of the soil, including nutrient cations. This study used Raman microspectroscopy to study how nutrient cations affect alkanes' bioavailability to Acinetobacter baylyi ADP1 (a known degrader). Treated with Na, K, Mg, and Ca at 10 mM, A. baylyi was exposed to seven n-alkanes (decane, dodecane, tetradecane, hexadecane, nonadecane, eico-sane, and tetracosane) and one alkane mixture (mineral oil). Raman spectral analysis indicated that bioavailability of alkanes varied with carbon chain lengths, and additional cations altered the bacterial response to n-alkanes. Sodium significantly increased the bacterial affinity toward decane and dodecane, and K and Mg enhanced the bioavailability of tetradecane and hexadecane. In contrast, the bacterial response was inhibited by Ca for all alkanes. Similar results were observed in mineral oil exposure. Our study employed Raman spectral assay to offer a deep insight into how nutrient cations affect the bioavailability of alkanes, suggesting that nutrient cations can play a key role in influencing the harmful effects of hydrocarbons and could be optimized to enhance the bioremediation strategy.