Characterization of the distribution of internal motions in the basic pancreatic trypsin inhibitor using a large number of internal NMR probes
Characterization of the distribution of internal motions in the basic pancreatic trypsin inhibitor using a large number of internal NMR probes
复制标题
使用大量内部 NMR 探针表征碱性胰蛋白酶抑制剂的内部运动分布
DOI：
10.1017/s0033583500004911
复制
发表时间：
1983
影响因子：
6.1
通讯作者：
G. Wagner
中科院分区：
文献类型：
--
作者：
G. Wagner
本文中描述的实验观察表明，在球状蛋白质中存在许多不同波动的分布。这些波动的特点是观察到许多天然的内部探针，如不稳定的肽质子和芳香族侧链。详细讨论了内探针发生反应所需的条件。通过开发和使用新的NMR技术促进了数据的结构解释，该技术提供了所有不稳定肽质子的共振的鉴定。通过NOE测量，获得了相关和不相关交换事件之间的区别。这使我们能够阐明在广泛的p2H和温度范围内的交换机制，并根据其寿命对不同的波动子集进行分类。进一步证明，外部条件如温度、p2H或压力的变化可以改变蛋白质中波动的分布。负责内部芳香族侧链的旋转机制也被发现随温度的变化，这些波动的机械方面进行了讨论。
The experimental observations described in this article indicated that a distribution of many different fluctuations is present in a globular protein. These fluctuations were characterized by observation of many natural internal probes such as the labile peptide protons and the aromatic side chains. The conditions which are necessary to get reactions of the internal probes have been discussed in detail. The structural interpretation of the data was facilitated by the development and the use of new NMR techniques which provided the identification of the resonances of all the labile peptide protons. With NOE measurements a distinction between correlated and uncorrelated exchange events was obtained. This enabled us to elucidate the exchange mechanism over a wide range of p2H and temperature and to classify different subsets of fluctuations with respect to their lifetimes. It was further demonstrated that a change of external conditions such as temperature, p2H or pressure can change the distribution of fluctuations in the protein. The mechanisms responsible for rotation of internal aromatic side chains were also found to change with temperature, and mechanistic aspects of these fluctuations were discussed.
影响因子：
2.9
作者：
BEECE, D;EISENSTEIN, L;YUE, KT
通讯作者：
YUE, KT
胰蛋白酶抑制剂中的氢交换率与尿素的热稳定性不相关。
影响因子：
2.9
作者：
Hilton,BD;Trudeau,K;Woodward,CK
通讯作者：
Woodward,CK
脯氨酸残基在牛胰蛋白酶抑制剂衍生物 RCAM(14-38) 折叠动力学中的作用。
影响因子：
5.6
作者：
Jullien,M;Baldwin,RL
通讯作者：
Baldwin,RL