Laponite nanoparticle-associated silated hydroxypropylmethyl cellulose as an injectable reinforced interpenetrating network hydrogel for cartilage tissue engineering
Laponite nanoparticle-associated silated hydroxypropylmethyl cellulose as an injectable reinforced interpenetrating network hydrogel for cartilage tissue engineering
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DOI：
10.1016/j.actbio.2017.11.027
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发表时间：
2018-01-01
影响因子：
9.7
通讯作者：
Rethore, Gildas
中科院分区：
文献类型：
--
作者：
Boyer, Cecile;Figueiredo, Lara;Rethore, Gildas
关节软骨是一种结缔组织，不会自发愈合。为了解决这个问题，生物材料辅助细胞治疗已经研究了有希望的进展。尽管在三维支架方面取得了重大进展，但缺乏强机械性能仍然是一个问题。这篇文章的目的是开发一种复合水凝胶，使用少量的纳米增强粘土称为laponites。这些锂皂石能够在硅化羟丙基甲基纤维素（Si-HPMC）水凝胶的凝胶结构内自固化。将Laponites（XLG）与Si-HPMC混合制备复合水凝胶，从而形成杂化互穿网络。这种互穿网络增加了水凝胶的机械性能。体外研究表明，交联后，XLG在复合材料内的细胞相容性或氧扩散方面没有副作用。在裸鼠皮下袋中植入6周期间，研究了含有复合水凝胶和软骨细胞的混合支架在体内形成软骨组织的能力。复合结构的组织学分析揭示了软骨样组织的形成，其具有含有糖胺聚糖和胶原的细胞外基质。总的来说，这种新的混合结构展示了一种互穿网络，其增强了水凝胶的机械性能，而不干扰其细胞相容性、氧扩散或软骨细胞在簇中自组织并产生细胞外基质组分的能力。这种复合水凝胶可能与治疗关节软骨缺损的大型动物模型中的软骨缺损有关。(c)2017由Elsevier Ltd代表Acta Materialia Inc.发布。
Articular cartilage is a connective tissue which does not spontaneously heal. To address this issue, biomaterial-assisted cell therapy has been researched with promising advances. The lack of strong mechanical properties is still a concern despite significant progress in three-dimensional scaffolds. This article's objective was to develop a composite hydrogel using a small amount of nano-reinforcement clay known as laponites. These laponites were capable of self-setting within the gel structure of the silated hydroxypropylmethyl cellulose (Si-HPMC) hydrogel. Laponites (XLG) were mixed with Si-HPMC to prepare composite hydrogels leading to the development of a hybrid interpenetrating network. This interpenetrating network increases the mechanical properties of the hydrogel. The in vitro investigations showed no side effects from the XLG regarding cytocompatibility or oxygen diffusion within the composite after cross-linking. The ability of the hybrid scaffold containing the composite hydrogel and chondrogenic cells to form a cartilaginous tissue in vivo was investigated during a 6-week implantation in subcutaneous pockets of nude mice. Histological analysis of the composite constructs revealed the formation of a cartilage-like tissue with an extracellular matrix containing glycosaminoglycans and collagens. Overall, this new hybrid construct demonstrates an interpenetrating network which enhances the hydro gel mechanical properties without interfering with its cytocompatibility, oxygen diffusion, or the ability of chondrogenic cells to self-organize in the cluster and produce extracellular matrix components. This composite hydrogel may be of relevance for the treatment of cartilage defects in a large animal model of articular cartilage defects. (c) 2017 Published by Elsevier Ltd on behalf of Acta Materialia Inc.