ANTIFUNGAL HYDROLASES IN PEA TISSUE .2. INHIBITION OF FUNGAL GROWTH BY COMBINATIONS OF CHITINASE AND BETA-1,3-GLUCANASE
ANTIFUNGAL HYDROLASES IN PEA TISSUE .2. INHIBITION OF FUNGAL GROWTH BY COMBINATIONS OF CHITINASE AND BETA-1,3-GLUCANASE
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DOI：
10.1104/pp.88.3.936
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发表时间：
1988-11-01
期刊：
影响因子：
7.4
通讯作者：
BOLLER, T
中科院分区：
文献类型：
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作者：
MAUCH, F;MAUCHMANI, B;BOLLER, T
几丁质酶和β-从豌豆荚中纯化的1，3-葡聚糖酶在真菌细胞壁的降解中起协同作用。通过将蛋白质制剂加入到置于含有萌发的真菌孢子的琼脂平板上的纸盘中，直接研究了这两种酶的抗真菌潜力。来自感染Fusarium solani f. sp. phaseoli的豌豆豆荚的蛋白质提取物，其含有高活性的几丁质酶和β-几丁质酶。1，3-葡聚糖酶，抑制生长的15个测试的18种真菌。来自未感染豌豆荚的蛋白质提取物，其含有低活性的几丁质酶和β-葡聚糖酶。1，3-葡聚糖酶，不抑制真菌生长。纯化的几丁质酶和β-单独测试的1，3-葡聚糖酶不抑制大多数测试真菌的生长。只有绿色木霉被单独的几丁质酶抑制，只有茄病镰刀菌豌豆专化型被β-单独的1，3-葡聚糖酶。然而，纯化的几丁质酶和β-几丁质酶的组合不适合于本发明。1，3-葡聚糖酶抑制所有真菌测试有效的粗蛋白提取物含有相同的酶活性。豌豆病原体Fusarium solani f. sp. pisi和豌豆的非病原体Fusarium solani f. sp. phaseoli同样被几丁质酶和β-葡萄糖苷酶强烈抑制。1，3-葡聚糖酶，表明两种真菌的差异致病性不是由于豌豆酶的差异敏感性。真菌生长的抑制是由菌丝顶端的裂解引起的。
Chitinase and .beta.-1,3-glucanase purified from pea pods acted synergistically in the degradation of fungal cell walls. The antifungal potential of the two enzymes was studied directly by adding protein preparations to paper discs placed on agar plates containing germinated fungal spores. Protein extracts from pea pods infected with Fusarium solani f.sp. phaseoli, which contained high activities of chitinase and .beta.-1,3-glucanase, inhibited growth of 15 out of 18 fungi tested. Protein extracts from uninfected pea pods, which contained low activities of chitinase and .beta.-1,3-glucanase, did not inhibit fungal growth. Purified chitinase and .beta.-1,3-glucanase, tested individually, did not inhibit growth of most of the test fungi. Only Trichoderma viride was inhibited by chitinase alone, and only Fusarium solani f.sp. pisi was inhibited by .beta.-1,3-glucanase alone. However, combinations of purified chitinase and .beta.-1,3-glucanase inhibited all fungi tested as effectively as crude protein extracts containing the same enzyme activities. The pea pathogen, Fusarium solani f.sp. pisi, and the nonpathogen of peas, Fusarium solani f.sp. phaseoli, were similarly strongly inhibited by chitinase and .beta.-1,3-glucanase, indicating that the differential pathogenicity of the two fungi is not due to differential sensitivity to the pea enzymes. Inhibition of fungal growth was caused by the lysis of the hyphal tips.