Analysis of L-alanine:2-oxoglutarate aminotransferase isozymes in maize.
Analysis of L-alanine:2-oxoglutarate aminotransferase isozymes in maize.
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玉米中 L-丙氨酸：2-酮戊二酸转氨酶同工酶的分析。
DOI：
10.1007/bf00569328
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发表时间：
1992
影响因子：
2.4
通讯作者：
Sachs,MM
中科院分区：
文献类型：
--
作者：
Watson,NR;Peschke,VM;Russell,DA;Sachs,MM
玉米丙氨酸：2-酮戊二酸氨基转移酶（ALT）同工酶分析表明，有三个基因编码该酶的活性。两个基因产物相互作用形成异二聚体，而第三个基因产物不与其他两个相互作用。凝胶电泳和ALT染色后出现的另一种同工酶是谷氨酸脱氢酶-1。厌氧处理不会导致ALT水平升高，表明先前报道的由该处理引起的丙氨酸水平升高可能是由于丙酮酸（ALT的底物）水平升高。
Isozyme analysis ofl-alanine:2-oxoglutarate aminotransferase (ALT) in maize indicates that there are three genes encoding this enzyme activity. Two of the gene products interact with each other to form heterodimers, while the third gene product does not interact with the other two. Another isozyme that appears after gel electrophoresis and ALT staining is shown to be glutamate dehydrogenase-1. Anaerobic treatment does not result in increased ALT levels, indicating that the previously reported increase in alanine levels caused by this treatment may be due to increases in the level of pyruvate, a substrate of ALT.