Induction of 1-cys peroxiredoxin expression by oxidative stress in lung epithelial cells
Induction of 1-cys peroxiredoxin expression by oxidative stress in lung epithelial cells
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DOI：
10.1152/ajplung.00078.2003
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发表时间：
2003-08-01
影响因子：
4.9
通讯作者：
Fisher, AB
中科院分区：
文献类型：
--
作者：
Kim, HS;Manevich, Y;Fisher, AB
1- cyys过氧化物还氧蛋白（1-cysPrx）是过氧化物还氧蛋白家族的一员，含有一个保守的半胱氨酸残基，可以减少广谱的氢过氧化物。我们研究了1-cysPrx在高氧、H2O2或百草枯引起的氧化应激下在大鼠肺和肺细胞系中的表达变化。高氧60 h （>95% O-2）后，大鼠肺1-cysPrx mRNA和蛋白水平及过氧化物酶活性较对照组显著升高1.5 ~ 2倍（P < 0.05）。在暴露于O-2 63或72小时后，在小鼠肺部观察到类似的1-cysPrx诱导；小鼠肺部的酶诱导在野生型和谷胱甘肽过氧化物酶1基因靶向小鼠中相似。H2O2和百草枯处理诱导L2细胞1-cysPrx基因表达。经Trolox或n -乙酰半胱氨酸预处理后，酶诱导减弱。放线菌素D处理表明，在H2O2或百草枯存在下，1-cysPrx mRNA的稳定性没有改变，表明氧化应激下表达的增加在转录水平上受到调控。这些数据表明，抗氧化酶1-cysPrx是由氧化应激诱导的肺细胞。
1-Cys peroxiredoxin (1-cysPrx), a member of the peroxiredoxin family that contains a single conserved cysteine residue, reduces a broad spectrum of hydroperoxides. We studied changes in 1-cysPrx expression in rat lungs and lung cell lines in response to oxidative stress due to hyperoxia, H2O2, or paraquat. After 60 h of hyperoxia (>95% O-2), mRNA and protein levels of 1-cysPrx and peroxidase activity were significantly elevated in rat lungs by similar to1.5- to 2-fold compared with the control (P < 0.05). A similar induction of 1-cysPrx was observed in mouse lungs following exposure to O-2 for 63 or 72 h; enzyme induction in mouse lungs was similar for wild-type and glutathione peroxidase 1 gene-targeted mice. H2O2 and paraquat treatment induced 1-cysPrx gene expression in L2 cells. Enzyme induction was attenuated by pretreatment with Trolox or N-acetylcysteine. Actinomycin D treatment showed that stability of 1-cysPrx mRNA was not altered in the presence of H2O2 or paraquat, indicating that increased expression with oxidative stress is regulated at the transcriptional level. These data indicate that the antioxidant enzyme 1-cysPrx is induced in lung cells by oxidative stress.