The structure of the membrane systems in a novel muscle cell modified for heat production.
The structure of the membrane systems in a novel muscle cell modified for heat production.
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DOI：
10.1083/jcb.107.3.1099
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发表时间：
1988-09
期刊：
影响因子：
--
通讯作者：
Franzini-Armstrong C
中科院分区：
文献类型：
--
作者：
Block BA;Franzini-Armstrong C
一种产热器官，由眼肌改造而来，为几种海洋鱼类的大脑和眼睛提供温暖。与产热相关的眼外肌由结构上不同于先前描述的所有其他类型的肌肉的经修饰的肌细胞组成。在“加热器”细胞中，收缩丝几乎不存在，细胞体积充满线粒体和光滑的膜。冷冻断裂的研究和负染色的微粒体组分与钒酸盐处理表明，大多数的膜系统的加热细胞具有高的Ca 2 +-ATP酶密度，相当于骨骼肌肌浆网（SR）。高电压电子显微照片的加热器细胞渗透与高尔基染色表明，细胞也有广泛的横小管系统与复杂的三维结构。横小管和SR之间的连接区域发生在加热细胞中，并含有足蛋白。加热细胞中产热的激活可能通过参与兴奋-收缩偶联的相同蛋白质组分发生，并且似乎与SR处的ATP依赖性钙循环相关。
A thermogenic organ, modified from an eye muscle, warms the brain and eyes of several oceanic fish. The extraocular muscles associated with thermogenesis are composed of modified muscle cells that are structurally distinct from all other types of muscle previously described. In "heater" cells, contractile filaments are virtually absent and the cell volume is packed with mitochondria and smooth membranes. Freeze-fracture studies and negative staining of microsomal fractions treated with vanadate indicate that most of the membrane system of heater cells has a high Ca2+-ATPase density and is equivalent to skeletal muscle sarcoplasmic reticulum (SR). High voltage electron micrographs of heater cells infiltrated with the Golgi stain demonstrate that the cells also have an extensive transverse tubule system with a complicated three-dimensional structure. Junctional regions between transverse tubules and SR occur in the heater cell and contain feet protein. Activation of thermogenesis in heater cells may occur through the same protein components involved in excitation- contraction coupling and appears to be associated with the ATP- dependent cycling of calcium at the SR.