Disposition of Charged Nanoparticles after Their Topical Application to the Skin
Disposition of Charged Nanoparticles after Their Topical Application to the Skin
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DOI：
10.1159/000270381
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发表时间：
2010-01-01
影响因子：
2.7
通讯作者：
Guy, R. H.
中科院分区：
文献类型：
--
作者：
Wu, X.;Landfester, K.;Guy, R. H.
本初步研究的目的是检查局部应用后带电荷的纳米颗粒在皮肤上和皮肤内的分布情况，并探讨该配方是否具有局部递送相关“活性”物质的潜在效用。三个纳米粒子(大约。（直径为100 nm）：阳离子氨基功能化聚苯乙烯、阴离子羧基功能化聚苯乙烯和阴离子聚（l-丙交酯），其中每个都加入了荧光基团N-（2,6-二异丙基苯基）苝-3,4-二碳亚胺（PMI）。将这些配方应用于离体切除的猪皮肤6小时。在处理后清洁皮肤表面后，用激光扫描共聚焦显微镜检查皮肤，或反复剥离胶带，随后分析去除的角质层（SC）是否存在PMI。阳离子纳米颗粒对带负电的皮肤表面表现出明显的亲和力（与阴离子载体相反），并向SC输送了大量的模型“活性”剂（PMI）。版权所有(C) 2009 S. kager AG, Basel
The objective of this preliminary investigation was to examine the disposition of charged nanoparticles on and within the skin following their topical application and to explore whether the formulations have potential utility for the local delivery of an associated 'active' substance. Three nanoparticles (approx. 100 nm in diameter) were investigated: cationic amino-functionalized polystyrene, an anionic carboxyl-functionalized polystyrene and anionic poly-(L-lactide), into each of which the fluorophore N-(2,6-diisopropylphenyl) perylene-3,4-dicarboximine (PMI) was incorporated. Formulations were applied to excised porcine skin in vitro for 6 h. After cleaning the skin surface following treatment, the skin was either examined by laser scanning confocal microscopy or subjected to repeated tape-stripping and subsequent analysis of the removed stratum corneum (SC) for the presence of PMI. The cationic nanoparticles showed clear affinity for the negatively charged skin surface (in contrast to the anionic carriers) and delivered a significantly greater amount of the model 'active' agent (PMI) into the SC. Copyright (C) 2009 S. Karger AG, Basel