A NEW AND SENSITIVE METHOD FOR MEASURING THERMAL NOCICEPTION IN CUTANEOUS HYPERALGESIA
A NEW AND SENSITIVE METHOD FOR MEASURING THERMAL NOCICEPTION IN CUTANEOUS HYPERALGESIA
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DOI：
10.1016/0304-3959(88)90026-7
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发表时间：
1988-01-01
期刊：
影响因子：
7.4
通讯作者：
JORIS, J
中科院分区：
文献类型：
--
作者：
HARGREAVES, K;DUBNER, R;JORIS, J
描述了一种测量无约束动物对热刺激的皮肤痛觉过敏的方法。测试范例使用行为终点的自动检测;重复测试并不有助于观察到的痛觉过敏的发展。与盐水处理的爪相比，角叉菜胶诱导的炎症导致显著更短的爪缩回潜伏期，并且这些潜伏期变化对应于降低的热伤害性阈值。热方法和Randall-Selitto机械方法检测到剂量相关的痛觉过敏和吗啡或吲哚美辛的阻断。然而，热方法显示出更大的生物测定灵敏度，并允许测量其他行为参数，除了伤害性阈值。
A method to measure cutaneous hyperalgesia to thermal stimulation in unrestrained animals is described. The testing paradigm uses an automated detected of the behavioral end-point; repeated testing does not contribute to the development of the observed hyperalgesia. Carrageenan-induced inflammation resulted in significantly shorter paw withdrawal latencies as compared to saline-treated paws and thse latency changes corresponded to a decreased thermal nociceptive threshold. Both the thermal method and the Randall-Selitto mechanical method detected dose-related hyperalgesia and its blockade by either morphine or indomethacin. However, the thermal method showed greater bioassay sensitivity and allowed for the measurement of other behavioral parameters in addition to the nociceptive threshold.