Genome imprinting regulated by the mouse Polycomb group protein Eed
Genome imprinting regulated by the mouse Polycomb group protein Eed
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DOI：
10.1038/ng1125
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发表时间：
2003-04-01
期刊：
影响因子：
30.8
通讯作者：
Magnuson, T
中科院分区：
文献类型：
--
作者：
Mager, J;Montgomery, ND;Magnuson, T
表观遗传调控对于时间、组织特异性和来源依赖性的基因表达是必不可少的。最近发现，小鼠Polycomb组（PcG）基因Eed（胚胎外胚层发育）的作用，以维持镇压的印记X染色体。在这里，我们调查是否EED也需要调节常染色体印迹基因座。表达分析表明，成绩单从沉默等位基因的一个子集的父系抑制基因存在于Eed（-/-）胚胎。在这些胚胎中保留了起源的父母甲基化，但我们观察到在受影响而不是未受影响的基因座的差异甲基化区域（DMR）中特定CpG的甲基化状态的变化。这些数据确定Eed作为一类新的反式作用因子的成员，调节父母的起源在印迹基因座的表达。
Epigenetic regulation is essential for temporal, tissue-specific and parent-of-origin-dependent gene expression. It has recently been found that the mouse Polycomb group (PcG) gene Eed (embryonic ectoderm development) acts to maintain repression of the imprinted X chromosome. Here, we investigated whether Eed is also required for regulation of autosomal imprinted loci. Expression analyses showed that transcripts from the silent alleles of a subset of paternally repressed genes were present in Eed(-/-) embryos. Parent-of-origin methylation was preserved in these embryos, but we observed changes in the methylation status of specific CpGs in differentially methylated regions (DMRs) at affected but not at unaffected loci. These data identify Eed as a member of a new class of trans-acting factors that regulate parent-of-origin expression at imprinted loci.