Considerations on the possibilities and limitations of comprehensive normal phase-reversed phase liquid chromatography (NPLC x RPLC)
Considerations on the possibilities and limitations of comprehensive normal phase-reversed phase liquid chromatography (NPLC x RPLC)
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DOI：
10.1002/jssc.200500451
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发表时间：
2006-03-01
影响因子：
3.1
通讯作者：
Sandra, P
中科院分区：
文献类型：
--
作者：
François, I;de Villiers, A;Sandra, P
A comprehensive normal phase system LC-reversed phase LC (NPLC X RPLC) was evaluated for the separation of a pharmaceutical Mixture and citrus oil extracts. NPLC was performed on a 25 cm x 1 mm ID x 5 mu m d(p) diol phase. In the second dimension, an RP 18 monolithic column (10 cm L x 4.6 mm ID x 2 mu m macropore size) and an octadecyl silicagel-packed column (5 cm L x 4.6 mm ID x 3.5 mu m d(p)) were applied for the analyses of the pharmaceutical sample and the citrus oil extracts. respectively. A two-position/ten-port switching valve was used as interface. Under optimised LC conditions, the high degree of orthogonality between NP and RP resulted in peak capacities of 300 for the pharmaceutical sample and of 450 for the citrus oil extract composed of lemon and orange oil. Despite the features of NPLC x RPLC, several shortcomings related with the solvent incompatibility between the two LC modes were identified and the practical consequences were discussed.
