Distribution and Antioxidant Activity of Free and Bound Phenolic Acids in Sugarcane Tissues
Distribution and Antioxidant Activity of Free and Bound Phenolic Acids in Sugarcane Tissues
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甘蔗组织中游离酚酸和结合酚酸的分布及其抗氧化活性
DOI：
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发表时间：
2014
影响因子：
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通讯作者：
Yu, Shu-Juan
中科院分区：
文献类型：
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作者：
Zhou, Hui-Fang;Li, Chao;Zhao, Zhen-Gang;Yu, Shu-Juan
为了探讨甘蔗组织中酚酸的不同存在形式，采用反相高效液相色谱法（RP-HPLC）对甘蔗汁、甘蔗叶和甘蔗渣中游离和结合态酚酸进行了定性和定量分析。结果表明，酚酸类物质在甘蔗中主要以结合态存在，但在不同组织中含量和存在形式不同。甘蔗叶和甘蔗渣中酚酸含量高于蔗汁。蔗汁、蔗叶和甘蔗渣中游离酚酸含量分别为317.18 mg/kg（干基）、1568.37 mg/kg（干基）和1504.57 mg/kg（干基）。蔗汁、蔗叶和甘蔗渣中的结合酚酸含量分别为740.32 mg/kg（干基）、2725.41 mg/kg（干基）和2452.13 mg/kg（干基）。在甘蔗汁中，咖啡酸和没食子酸主要以游离形式存在，而阿魏酸和香豆酸主要以结合形式存在。咖啡酸、阿魏酸和香豆酸主要以结合形式存在于甘蔗叶和甘蔗渣中。在甘蔗不同组织中，游离酚酸对DPPH和ABTS的清除活性优于结合酚酸。甘蔗组织中游离酚酸和结合酚酸含量与抗氧化活性呈正相关。
In order to explore the different forms of phenolic acids in the tissues of sugarcane, qualitative and quantitative analyses of free and bound phenolic acids in the cane juice, leaves, and bagasse of sugarcane were performed using reversed-phase high-performance liquid chromatography(RP-HPLC). The results showed that phenolic acids primarily existed in the bound form in sugarcane; however, the content and form were different in the three different tissues. The leaves and bagasse had higher phenolic acid content than cane juice. The free phenolic acid content in cane juice, leaves, and bagasse was 317.18 mg/kg(dry solids), 1568.37 mg/kg(dry basis), and 1504.57 mg/kg(dry basis), respectively. The bound phenolic acid content in cane juice, leaves and bagasse was 740.32 mg/kg(dry solids), 2725.41 mg/kg(dry basis), and 2452.13 mg/kg(dry basis). In cane juice, caffeic acid and gallic acid were primarily in the free form, whereas ferulic acid and coumaric acid were primarily in the bound form. Caffeic acid, ferulic acid, and coumaric acid were primarily in the bound form in cane leaves and bagasse. In the different sugarcane tissues, free phenolic acids exhibited better free-radical removal activities towards DPPH and ABTS than bound phenolic acids. The free and bound phenolic acid content in sugarcane tissues was positively correlated with antioxidant activity.