A comparative study of conventional versus new, magnesium-poor Vacutainer® Sodium Citrate blood collection tubes for determination of prothrombin time and INR
A comparative study of conventional versus new, magnesium-poor Vacutainer® Sodium Citrate blood collection tubes for determination of prothrombin time and INR
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DOI：
10.1016/j.thromres.2014.04.016
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发表时间：
2014-07-01
影响因子：
7.5
通讯作者：
Cobbaert, Christa M.
中科院分区：
文献类型：
--
作者：
van den Besselaar, Anton M. H. P.;van Vlodrop, Iris J. H.;Cobbaert, Christa M.
引言：常规的真空采血管（Vacutainer®）枸橼酸钠血液采集管含有相对高浓度的污染性镁离子，这可能导致凝血酶原时间（PT）和国际标准化比值（INR）缩短。近期，Vacutainer®枸橼酸钠管的制造商推出了一种新的带有贫镁塞的采血管。新型低镁管中的镁浓度明显低于常规塑料管。本研究的目的是比较使用新型管采集的标本与使用常规管采集的标本所测定的PT和INR。
材料与方法：使用常规的Vacutainer®枸橼酸钠管和新型的低镁Vacutainer®管，从22名健康人和65名接受维生素K拮抗剂治疗的患者采集静脉血标本。使用四种凝血活酶试剂测定PT和INR，即三个品牌的重组人组织因子和一个品牌的复合兔脑试剂。用比色法测定枸橼酸盐血浆中的镁浓度。
结果：当使用三种重组人凝血活酶试剂时，两种采血管之间的PT、INR和国际敏感指数（ISI）差异显著，但使用兔凝血活酶时差异不显著。采血管之间的PT和INR差异与镁浓度差异相关（P < 0.001）。对于使用常规管采集的标本，四种试剂之间的INR偏差大于使用新型管采集的标本的INR偏差。
结论：使用贫镁管可提高试剂之间INR的一致性。（c）2014爱思唯尔有限公司。保留所有权利。
Introduction: Conventional Vacutainer (R) Sodium Citrate blood collection tubes contain a relatively high concentration of contaminating magnesium ions, which may result in shortening of the prothrombin time (PT) and the International Normalized Ratio (INR). Recently the manufacturer of Vacutainer (R) Sodium Citrate tubes introduced new tubes with a magnesium-poor stopper. The magnesium concentration in the new low-Mg tubes is significantly lower than that in the conventional plastic tubes. The purpose of the present study was to compare PT and INR determined in specimens drawn with the new tubes to those drawn with the conventional tubes.Materials and Methods: Venous blood specimens were collected from 22 healthy persons and 65 patients treated with vitamin K-antagonists using conventional Vacutainer (R) Sodium Citrate tubes and new, low-Mg Vacutainer (R) tubes. PT and INR were determined with four thromboplastin reagents,i.e., three brands of recombinant human tissue factor and one brand of combined rabbit brain reagent. Magnesium concentrations were determined in the citrate plasmas with a colorimetric method.Results: The differences in PT, INR and International Sensitivity Index (ISI) between the two tubes were significant when using three recombinant human thromboplastin reagents, but were not significant when using the rabbit thromboplastin. The PT and INR differences between the tubes correlated with the magnesium concentration differences (P < 0.001). The INR bias between the four reagents was greater for specimens drawn with conventional tubes than the INR bias for specimens drawn with the new tubes.Conclusion: Agreement of INR between reagents is improved by using magnesium-poor tubes. (c) 2014 Elsevier Ltd. All rights reserved.