Computer programs for the analysis of immunohistochemically labeled spinal cord sections.
Computer programs for the analysis of immunohistochemically labeled spinal cord sections.
复制标题
用于分析免疫组织化学标记的脊髓切片的计算机程序。
DOI：
10.1016/0165-0270(90)90156-a
复制
发表时间：
1990
影响因子：
3
通讯作者：
Bullitt,E
中科院分区：
文献类型：
--
作者：
Johnson,EM;Bullitt,E
本文介绍了一种用于神经组织免疫组织化学标记密度分析的微机图象处理系统。该系统允许跟踪在数字化图像上看到的解剖区域，并确定位于每个定义区域内的所有像素的面积和平均灰度级。校正不均匀的屏幕照明和非线性的视频摄像机的响应，在光密度梯度。标准化程序确保在每个实验期间使用相同水平的入射照明。描述了该系统的应用的一个例子，其中确定在啮齿动物脊髓部分脊髓损伤后免疫组化染色密度的单侧变化。该系统提供了一个敏感和客观的手段来分析这种变化。
A microcomputer based image processing system has been developed to analyze the density of immunohistochemical labeling within neural tissue. The system permits the tracing of anatomical regions seen on a digitized image and the determination of the area and the mean gray level of all pixels lying within each defined region. Corrections are made for uneven screen illumination and for nonlinearity of the video camera's response to gradations in optical density. A standardization procedure ensures that the same level of incident illumination is used during each experiment. An example of an application of this system is described in which unilateral changes in immunohistochemical staining density were determined within the rodent spinal cord following partial spinal injury. This system provides a sensitive and objective means of analyzing such changes.