AvrXa3: A novel member of avrBs3 gene family from Xanthomonas oryzae pv. oryzae has a dual function
AvrXa3: A novel member of avrBs3 gene family from Xanthomonas oryzae pv. oryzae has a dual function
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DOI：
10.1080/10020070412331344311
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发表时间：
2004-09
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影响因子：
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通讯作者：
Ping Li;Juying Long;Y. Huang;Yan Zhang;Jinsheng Wang
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文献类型：
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作者：
Ping Li;Juying Long;Y. Huang;Yan Zhang;Jinsheng Wang
摘要从黄单胞菌致病性黄单胞菌3号生理小种Jxo Ⅲ的cDNA文库中筛选到两个阳性克隆pUAV45和pUAV47。日本系统中的染料（Xoo），使用基于Tn5的技术。pUAV45克隆含有25.4kb Xoo基因组DNA插入片段。以avrXa10为探针进行Southern杂交分析，结果表明pUAV45中插入片段与avrX10具有同源性。此外，在Xoo插入片段内，通过与avrXa10杂交鉴定出较小的5.7 kb Kpn I片段（pUAV5k）。将pUAV45和pUAV5k导入Pxo99菌株（菲律宾系统6号生理小种）后，Pxo99对水稻品种早濑爱国3号（Xa3）的致病力降低，对水稻品种Cas209（Xa10）的致病力增强。序列分析结果表明，在5.7kb的Kpn 1基因片段（pUAV5k）中有一个2598 bp的开放阅读框（ORF）。该ORF与avrXa10具有较高的同源性（97%）。该基因的ORF序列由8.5个34个氨基酸的串联重复单元组成，一个亮氨酸拉链（Leucine zipper，L.
Abstract Two positive clones pUAV45 and pUAV47 were identified from the cDNA library of Jxo III, a race 3 strain of Xanthomonas oryzae pv. oryzae Dye (Xoo) in Japanese system, using Tn5 based technology. pUAV45 clone contained a 25.4 kb Xoo genomic DNA insert. Southern blot analysis with avrXa10 as the probe showed that DNA insert in pUAV45 shares homology with avrX10. Furthermore within the Xoo insert, a smaller 5.7 kb KpnI fragment (pUAV5k) was identified through hybridization with avrXa10. The transformation of pUAV45 and pUAV5k into the strain Pxo99 (race 6 in Philippine system) led to the decrease of Pxo99 pathogenicity on rice cultivar Wase Aikoku 3 (Xa3) and the increase of the pathogen pathogenicity on Cas209 (Xa10). The result of sequence analysis showed that there is a 2598 bp open reading frame (ORF) within the 5.7 kb Kpn1 fragment (pUAV5k). The ORF shared high identity (97%) with avrXa10. The deduced sequence of the ORF contained 8.5 tandem repeat units of 34-amino-acids, one leucine zipper (L...